Dispersive solid-phase imprinting of proteins for the production of plastic antibodies

Publication year: 2018
Authors: Ashley J., Feng X., Halder A., Zhou T., Sun Y.*

Department of Micro and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Lyngby, Denmark
* corresponding author

Published in: Chemical Communications, 2018, Vol. 54 (27), p. 3355-3358
doi: 10.1039/c8cc00343b

We describe a novel dispersive solid-phase imprinting technique for the production of nano-sized molecularly imprinted polymers (nanoMIPs) as plastic antibodies. The template was immobilized on in-house synthesized magnetic microspheres instead of conventional glass beads. As a result, high-affinity and template-free MIPs were produced in higher yields.

MP-SPR keywords: affinity, b-lactoglobulin, binding, hemoglobin, molecularly imprinted polymers, protein – nanoMIP interactions, RNase, trypsin