Homogeneous cellulose thin films by regeneration of cellulose xanthate: properties and characterization

Publication year: 2017
Authors: Weißl M. 1, 2, Niegelhell K. 2, Reishofer D. 2, Zankel A. 3, Innerlohinger J. 4, Spirk S. 2
Affiliations:

1 - Institute for Chemistry and Technology of Materials, Graz University of Technology, Graz, Austria
2 - Institute for Paper, Pulp and Fibre Technology, Graz University of Technology, Graz, Austria
3 - Institute for Electron Microscopy and Nanoanalysis, NAWI Graz, Graz University of Technology and Centre for Electron Microscopy, Graz, Austria
4 - Lenzing AG, Lenzing, Austria

Published in: Cellulose, 2017, Vol. 25, Issue 1, p. 711-721
doi: 10.1007/s10570-017-1576-3

The preparation and characterization of cellulose thin films derived from cellulose xanthate is reported. The films are prepared by depositing alkaline aqueous solutions of cellulose xanthate onto silicon wafers, followed by a spin coating step. Depending on the xanthate concentration used for spin coating, films with 50 and 700 nm thickness are obtained. The cellulose xanthate is converted to cellulose by exposing the films to HCl vapors over a period of 20 min. The conversion is monitored by ATR-IR spectroscopy, which allows for tracking the rupture of C–S and C=S bonds during the regeneration process. The conversion is accompanied by a reduction of the film thickness of ca 40% due to the removal of the bulky xanthate group. The films feature a homogenous, but porous morphology as shown by atomic force microscopy. Further, the films were investigated towards their interaction with Bovine Serum Albumin (BSA) and fibrinogen by means of multi-parameter surface plasmon resonance spectroscopy. Similar as other cellulose thin films BSA adsorption is low while fibrinogen adsorbs to some extent at physiological pH (7.4).


MP-SPR keywords: adsorbed mass, bovine serum albumin (BSA), cellulose thin films, fibrinogen, kinetics, protein binding on cellulose