Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance

Publication year: 2017
Authors: Mauricio E. Di Ianni 1, Germán A. Islan 2, Cecilia Y. Chain 3, Guillermo R. Castro 2, Alan Talevi 1, María E. Vela 3
Affiliations:

1 - Laboratorio de Investigación y Desarrollo de Bioactivos (LIDeB), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina
2 - Laboratorio de Nanobiomateriales, Centro de Investigación y Desarrollo de Fermentaciones Industriales (CINDEFI), UNLP-CONICET, CCT La Plata, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, La Plata, Argentina
3 - Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), CONICET-UNLP, CC 16 Suc 4, La Plata, 1900 Buenos Aires, Argentina

Published in: Journal of Nanomaterials, Vol. 2017, 11 pages
doi: 10.1155/2017/6509184

The applications of pharmaceutical and medical nanosystems are among the most intensively investigated fields in nanotechnology. A relevant point to be considered in the design and development of nanovehicles intended for medical use is the formation of the “protein corona” around the nanoparticle, that is, a complex biomolecular layer formed when the nanovehicle is exposed to biological fluids. The chemical nature of the protein corona determines the biological identity of the nanoparticle and influences, among others, the recognition of the nanocarrier by the mononuclear phagocytic system and, thus, its clearance from the blood. Recent works suggest that Surface Plasmon Resonance (SPR), extensively employed for the analysis of biomolecular interactions, can shed light on the formation of the protein corona and its interaction with the surroundings. The synthesis and characterization of solid lipid nanoparticles (SLN) coated with polymers of different chemical nature (e.g., polyvinyl alcohol, chitosans) are reported. The proof-of-concept for the use of SPR technique in characterizing protein-nanoparticle interactions of surface-immobilized proteins (immunoglobulin G and bovine serum albumin, both involved in the formation of the corona) subjected to flowing SLN is demonstrated for non-chitosan-coated nanoparticles. All assayed nanosystems show more preference for IgG than for BSA, such preference being more pronounced in the case of polyvinyl-alcohol-coated SLN.


MP-SPR keywords: BSA and IgG amino coupling on SAM surface, corona proteins, drug delivery nanoparticles, lipid nanoparticles, nanoparticle binding on surface immobilized protein