Publications: Life Science

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ORP2 interacts with phosphoinositides and controls the subcellular distribution of cholesterol

Publication year: 2019
Authors: Koponen A. a, Arora A. a, Takahashi K. b, Kentala H. a, Kivelä A.M. a, Jääskeläinen E. a, Peränen J. b, Somerharju P. c, Ikonen E. b, Viitala T. d, Olkkonen V.M. a,b
Published in: Biochimie, 2019, Vol. 158, p. 90-101
ORP2 is a sterol-binding protein with documented functions in lipid and glucose metabolism, Akt signaling, steroidogenesis, cell adhesion, migration and proliferation. [...]

Design and evaluation of a multiplexed angular-scanning surface plasmon resonance system employing line-laser optics and CCD detection in combination with multi-ligand sensor chips

Publication year: 2019
Authors: Lakayan D. a, Tuppurainen J. c, Suutari T.E. a,d, van Iperen D.J. e, Somsen G.W. a, Kool J. a
Published in: Sensors and Actuators B: Chemical, 2019, Vol. 282, p. 243-250
An angle-scanning Kretschmann configuration SPR instrument allowing multiplexed analysis is presented. Laser light was guided through optics that converted the collimated light into a line-shaped beam, which was [...]

Antimicrobial alumina nanobiostructures of disulfide- and triazole-linked peptides: Synthesis, characterization, membrane interactions and biological activity

Publication year: 2019
Authors: Torres L.M.F.C. a, Almeida M.T. a, Santos T.L. a, Marinho L.E.S. a, de Mesquita J.P. a, da Silva L.M. a, dos Santos W.T.P. b, Martins H.R. b, Kato K.C. b, Alves E.S.F. c, Liao L.M. c, .de Magalhães M.T.Q d, de Mendonça F.G. e, Pereira F.V. e, Resende J.M. e, Bemquerer M.P. f, Rodrigues M.A. g, Verly R.M. a
Published in: Colloids and Surfaces B: Biointerfaces , 2019, Vol. 177, p. 94-104
Due to the its physical-chemical properties, alumina nanoparticles have potential applications in several areas, such as nanobiomaterials [...]

Handbook of MP-SPR

Publication year: 2019
Authors: Marquart A., Kuncová-Kallio J., Albers M., Bombera R., Ståhlberg R.
Published in: Handbook of MP-SPR, 2019, Vol. 1
Handbook of Multi-Parametric Surface Plasmon Resonance for Molecular Interaction Analysis - Theory and Practice

Synergy between Zwitterionic Polymers and Hyaluronic Acid Enhances Antifouling Performance

Publication year: 2019
Authors: Yinqiang Xia,† Vahid Adibnia,‡ Cancan Shan,† Renliang Huang,§ Wei Qi,†,# Zhimin He,† Guojun Xie,∥ Mateusz Olszewski,∥ Gregory De Crescenzo,⊥ Krzysztof Matyjaszewski,*,∥ Xavier Banquy,*,‡ and Rongxin Su*,†,#
Published in: Langmuir
Challenges associated with nonspecific adsorption of proteins on sensor surfaces have steered the development of novel antifouling materials and strategies. Inspired by human synovial fluid composition and structure, we designed synergistic antifouling coatings with mixtures of hyaluronic acid (HA) and a zwitterionic bottlebrush polymer (BB). [...]

Real-Time Label-Free Targeting Assessment and in Vitro Characterization of Curcumin-Loaded Poly-lactic-co-glycolic Acid Nanoparticles for Oral Colon Targeting

Publication year: 2019
Authors: Mohamed A. Akl,†,‡ Alma Kartal-Hodzic,† Teemu Suutari,† Timo Oksanen,† Isabella Monia Montagner,§ Antonio Rosato,§,∥ Hatem R. Ismael,‡ Mohsen I. Afouna,‡ Paolo Caliceti,⊥ Marjo Yliperttula,†,⊥ Ahmed M. Samy,‡ Francesca Mastrotto,⊥ Stefano Salmaso,⊥ and Tapani Viitala*,†,#
Published in: ASC Omega
The exploitation of curcumin for oral disease treatment is limited by its low solubility, poor bioavailability, and low stability. Surface-functionalized poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) have shown promising results to ameliorate selective delivery of drugs to the gastrointestinal tract. [...]

Kinetic and thermodynamic insights into interaction of albumin with piperacillin: Spectroscopic and molecular modeling approaches

Publication year: 2019
Authors: Farzaneh Fathi a, Maryam Sharifi b, c, d, Amir Jafari e, Naser Kakavandi f, Soheila Kashanian g, h, Jafar Ezzati Nazhad Dolatabadi c, *, Mohammad-Reza Rashidi c, i, **
Published in: Journal of Molecular Liquids
The current study investigated the binding of BSA and piperacillin at multifarious concentrations (1 e128 mM) along with four different temperatures. To do this, three sensitive methods were employed including surface plasmon resonance (SPR), fluorescence spectroscopy and molecular docking (MD). [...]

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation

Publication year: 2019
Authors: Antonella Managò1,12, Valentina Audrito1,12, Francesca Mazzola2, Leonardo Sorci 3, Federica Gaudino1, Katiuscia Gizzi4, Nicoletta Vitale5, Danny Incarnato6, Gabriele Minazzato7, Alice Ianniello8, Antonio Varriale9, Sabato D’Auria9, Giulio Mengozzi8, Gianfranco Politano 10, Salvatore Oliviero 4,11, Nadia Raffaelli7,13 & Silvia Deaglio 1,13
Published in: Nature communications
Damage-associated molecular patterns (DAMPs) are molecules that can be actively or passively released by injured tissues and that activate the immune system. Here we show that nicotinate phosphoribosyltransferase (NAPRT), detected by antibody-mediated assays and mass spectrometry, is an extracellular ligand for Toll-like receptor 4 (TLR4) and a critical mediator of inflammation, acting as a DAMP. Exposure of human and mouse macrophages to NAPRT activates the inflammasome and NF-κB for secretion of inflammatory cytokines. [...]

Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials

Publication year: 2019
Authors: Sarah Dedisch 1,2 Annika Wiens 2 Mehdi D. Davari 2 Dominik Söder 1 Cesar Rodriguez‐Emmenegger 1,3 Felix Jakob 1,2 Ulrich Schwaneberg 1,2
Published in: Biotechnology and Bioengineerging
Enzyme immobilization is extensively studied to improve enzyme properties in catalysis and analytical applications. Here, we introduce a simple and versatile enzyme immobilization platform based on adhesion‐promoting peptides, namely Matter‐tags. [...]

Nanoparticles in Pharmacotheraphy Chapter 9B

Publication year: 2019
Authors: Barbara Jachimska
Published in: Elsevier
Physicochemical characterization of PAMAM dendrimer as a multifunctional nanocarriers

Phase-segregated Membrane Model assessed by a combined SPR-AFM Approach

Publication year: 2018
Authors: Millone D.A.M. 1, Vázquez R.F. 1, Maté S.M. 2, Vela M.E. 3
Published in: Colloids and Surfaces B: Biointerfaces, 2018, Vol. 172, p. 423-429
Model biomembranes can provide valuable insights into the properties of complex biological membranes. Among several techniques, Surface Plasmon Resonance (SPR) provides [...]

Molecular Details of Retinal Guanylyl Cyclase 1/GCAP-2 Interaction

Publication year: 2018
Authors: Rehkamp A. 1, Tänzler D. 1, Iacobucci C. 1, Golbik R.P. 2, Ihling C.H. 1, Sinz A. 1
Published in: Frontiers in Molecular Neuroscience, 2018, Vol. 11
The rod outer segment guanylyl cyclase 1 (ROS-GC1) is an essential component of photo-transduction in the retina. In the light-induced signal cascade, membrane-bound ROS-GC1 restores cGMP levels [...]

Affinity maturation and characterization of the ofatumumab monoclonal antibody

Publication year: 2018
Authors: Payandeh Z. 1, Rajabibazl M. 2,3, Mortazavi Y. 1,4, Rahimpour A. 3, Taromchi A.H. 1,4, Dastmalchi S. 5,6
Published in: Journal of Cellular Biochemistry, 2018
CD20 molecule, a phosphoprotein with 297 amino acids and four transmembrane domains, is a member of MS4A protein family. Anti-CD20 antibodies such as ofatumumab, which have been developed [...]

Hydrazone crosslinked hyaluronan-based hydrogels for therapeutic delivery of adipose stem cells to treat corneal defects

Publication year: 2018
Authors: Koivusalo L. a, Karvinen J. e, Sorsa E. b, Jönkkäri I. c, Väliaho J. b, Kallio P. b, Ilmarinen T. a, Miettinen S. a, Skottman H. a, Kellomäki M. a, b
Published in: Materials Science and Engineering: C, Vol. 85, 2018, p. 68–78
Corneal blindness is a worldwide problem, plagued by insufficient amount of high-quality donor tissue. Cell therapy using human adipose stem cells (hASCs) has risen as an alternative [...]

Characterization of G4 PAMAM dendrimer complexes with 5-fluorouracil and their interactions with bovine serum albumin

Publication year: 2018
Authors: Tokarczyk K., Jachimska B.
Published in: Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2018, Vol. 561, p. 357-363
The success of novel therapeutic strategies relies strongly on the development of a reliable active agent delivery mechanism. In this work, we address he question of how G4 PAMAM dendrimers [...]

Interaction of lecithin-cholesterol acyltransferase with lipid surfaces and apolipoprotein A-I derived peptides

Publication year: 2018
Authors: Casteleijn M. G., Parkkila P., Viitala T., Koivuniemi A.
Published in: Journal of Lipid Research, 2018
Lecithin-cholesterol acyltransferase (LCAT) is an enzyme responsible for the formation of cholesteryl esters from unesterified cholesterol (UE) and phospholipid (PL) molecules in high-density lipoprotein (HDL) particles. [...]

Membrane bound COMT isoform is an interfacial enzyme: general mechanism and new drug design paradigm

Publication year: 2018
Authors: Magarkar A. a,b, Parkkila P. a, Viitala T. a, Lajunen T. a, Mobarak E. c,d, Licari G. e, Cramariuc O. c, Vauthey E. e , Rógc T. d, Bunker A.* a
Published in: Chemical Communications, 2018, Vol. 54, Issue 28, p. 3440-3443
The enzyme catechol-O-methyltransferase (COMT) has water soluble (S-COMT) and membrane associated (MB-COMT), bitopic, isoforms. Of these MB-COMT is a drug target [...]

Topovectorial mechanisms control the juxtamembrane proteolytic processing of Nrf1 to remove its N-terminal polypeptides during maturation of the CNC-bZIP factor

Publication year: 2018
Authors: Xiang Y. 1, Halin J. 2, Fan Z. 1, Hu S. 1, Wang M. 1, Qiu L. 1, Zhang Z. 3, Mattjus P. 2, Zhang Y. 1
Published in: bioRxiv, 2018
The topobiological behaviour of Nrf1 dictates its post-translational modification and its ability to transactivate target genes. Here, we have elucidated that topovectorial mechanisms control [...]

Dispersive solid-phase imprinting of proteins for the production of plastic antibodies

Publication year: 2018
Authors: Ashley J., Feng X., Halder A., Zhou T., Sun Y.*
Published in: Chemical Communications, 2018, Vol. 54 (27), p. 3355-3358
We describe a novel dispersive solid-phase imprinting technique for the production of nano-sized molecularly imprinted polymers (nanoMIPs) as plastic antibodies. The template [...]

Bovine serum albumin binding study to erlotinib using surface plasmon resonance and molecular docking methods

Publication year: 2018
Authors: Taghipour P. 1, Zakariazadeh M. 2, Sharifi M. 3, Ezzati Nazhad Dolatabadi J. 4, Barzegar A. 5
Published in: Journal of Photochemistry and Photobiology B, 2018, Vol. 183, p. 11-15
Bovine serum albumin (BSA) is the most abundant protein in the blood circulation and it is commonly used for drug delivery in blood. Therefore, we aim to study BSA interaction with erlotinib [...]