Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor

Publication year: 2013
Authors: Chang Zeng (a), Xin Huang (a), Jiangmin Xu (a), Guifen Li (a), Jie Ma (a), Hai-Feng Ji (b), Shuifang Zhu (a), Hongjun Chen (a)
Affiliations:

a) Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, People’s Republic of China
b) Department of Chemistry, Drexel University, Philadelphia, PA 19010, USA

Published in: Analytical Biochemistry, Vol. 440 (2013), p. 18–22

We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maizechlorotic mottle virus (MCMV). 11-Mercaptoundecanoic acid was applied on a gold surface to form a self-assembled monolayer, and a layer of anti-MCMV antibody was crosslinked on the surface for speci?c recognition of MCMV. The effects of coupling reaction time and antibody concentration on detection sensitivity were studied. The coverage mass change is a function of the concentration of MCMV with adynamic range from 1 to 1000 ppb. The detection limit is approximately 1 ppb, which is approximately two orders of magnitude higher than that of the existing enzyme-linked immunosorbent assay (ELISA) method. The developed SPR sensor showed highly speci?c recognition for both puri?ed MCMV and crude extracts from real-world samples.


MP-SPR keywords: antibody, biosensor, concentration, crosslinking, virus detection