Refractive Index and Sensing of Glucose Molarities Determined using Au-Cr K-SPR at 670/785 nm Wavelenght

Publication year: 2019
Authors: P. Susthitha Menon*, Budi Mulyanti, Nur Akmar Jamil, Chandra Wulandari, Harbi Setyo Nugroho, Gan Siew Mei, Noor Faizah Zainul Abidin, Lilik Hasanah, Roer Eka Pawinanto & Dilla Duryha Berhanuddin

Universiti Kebangsaan Malaysia

Universitas Pendidikan Indonesia

Bandung Institute of Technology

Universiti Teknologi Malaysia




Published in: Sains Malaysiana 48(6)(2019): 1259–1265
doi: 10.17576/jsm-2019-4806-13

In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. The LbL film growth was monitored by surface plasmon resonance (SPR) by gold resonance angle shift analysis after each bilayer deposition. Subsequently, the films were applied as amperometric biosensors for dopamine detection using Prussian Blue (PB) as the electron mediator. The biosensor fabricated by MAO-B incorporated into liposomes composed of DPPG:POPG in the ratio (1:4) (w/w) showed the best performance with a sensitivity of 0.86 (μA cm− 2)/(mmol L− 1) and a detection limit of 0.33 mmol L− 1.

MP-SPR keywords: