A facile molecularly imprinted polymer-based fluorometric assay for detection of histamine

Publication year: 2018
Authors: Feng X., Ashley J., Zhou T., Halder A., Sun Y.
Affiliations:

Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, DK-2800 Kgs, Lyngby, Denmark

Published in: RSC Advances, 2018, Vol. 8, Issue 5, p. 2365
doi: 10.1039/c7ra11507e

Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, o-phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 μM with the limit of detection of 1.80 μM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85%.


MP-SPR keywords: affinity of nanoMIPs, food safety, histamine amino coupling chemistry on SAM, histamine detection, histamine on glass bead, nanoMIPs (200nm)