Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing

Publication year: 2018
Authors: Makhneva E. 1, 2, 6, Obrusnik A. 1, 2, 6, Farka Z. 3, Skladal P. 3, 4, Vandenbossche M. 5, Hegemann D. 5, Zajickova L. 1, 2
Affiliations:

1 - RG Plasma Technologies, Central European Institute of Technology, Masaryk University (CEITEC MU), Brno 621 00, Czechia
2 - Department of Physical Electronics, Faculty of Science, Masaryk University, Brno 611 37, Czechia
3 - RG Nanobiotechnology, CEITEC MU, Masaryk University, Brno 625 00, Czechia
4 - Department of Biochemistry, Faculty of Science, Masaryk University, Brno 625 00, Czechia
5 - Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland
6 - These authors contributed equally to this work

Published in: Japanese Journal of Applied Physics, 2018, Vol. 57, Number 1S, 01AG06
doi: 10.7567/JJAP.57.01AG06

Stable carboxyl-rich plasma polymers (PPs) were deposited onto the gold surface of surface plasmon resonance (SPR) chips under conditions that were chosen based on lumped kinetic model results. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity, allowing the formation of covalent linkages between biomolecules and a surface. Accordingly, the monoclonal antibody, specific to human serum albumin (HSA), was immobilized and the performance of SPR immunosensors was evaluated by the immunoassay flow test. The developed sensors performed high level of stability and provided selective and high response to the HSA antigen solutions. The achieved results confirmed that the presented methodologies for the grafting of biomolecules on the gold surfaces have great potential for biosensing applications.


MP-SPR keywords: amino coupling, anti-HSA, detection of human serum albumin, immunosensing, plasma polymers, polymer surface for antibody immobilization