Publications: Biosensors

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Electrochemical Proximity Assay of Platelet-Derived Growth Factor through Combined Surface Plasmon Resonance, Electrochemistry, and Fluorescence Spectroscopy Approach

Publication year: 2017
Authors: Zhou L. a, Chin B.A. a, Simonian A.L. b
Published in: ECS Transactions, 2017, Vol. 77, Issue 11, p. 1809-1814
Platelet-derived growth factor (PDGF) is a potential cancer marker, which plays an important role in early detection of cancers. Due to its trace amounts in blood sample, it is difficult to be detected. [...]

Label-free evaluation of carbon nanoparticles in Layer-by-Layer self-assembled enzyme-based biosensors

Publication year: 2017
Authors: David M. a, Florescu M. b, Barsan M. M. c, Brett M.A.C. c
Published in: Procedia Technology, Vo. 27, 2017, p. 304-305
The immobilization of biomolecules and the enhancement of the electron transfer are important factors regarding the performances of the enzyme-based biosensors. Layer-by-layer (LbL) strategies can be effectively used [...]

A Surface Plasmon Resonance Histamine Sensor Based on an Electropolymerized Molecularly Imprinted Polymer (E-MIP)

Publication year: 2017
Authors: Hubilla F.A.D., Mabilangan A.I., Advincula R.C., del Mundo F.R.
Published in: MATEC Web of Conferences, Vol. 95, 2017
In this paper, histamine sensing by surface plasmon resonance (SPR) spectroscopy using an electropolymerized molecularly imprinted polymer (E-MIP) of functionalized polyterthiophene and histamine is reported. [...]

Adsorption Studies of Organophosphonic Acids on Differently Activated Gold Surfaces

Publication year: 2016
Authors: Katrin Niegelhell †, Simon Leimgruber †‡, Thomas Grießer §, Christian Brandl ∥, Boril Chernev ∥, Robert Schennach ⊥, Gregor Trimmel †, and Stefan Spirk †
Published in: Langmuir, 2016, Vol. 32 (6), p. 1550–1559
In this study, the formation of self-assembled monolayers consisting of three organophosphonic acids (vinyl-, octyl-, and tetradecylphosphonic acid) from isopropanol solutions onto differently activated gold surfaces is studied in situ and in real time using multiparameter surface plasmon resonance (MP-SPR). [...]

Label-free C-reactive protein electronic detection with an electrolyte-gated organic field-effect transistor-based immunosensor

Publication year: 2016
Authors: Magliulo M. 1, De Tullio D. 2, Vikholm-Lundin I. 3, Albers W.M. 4, Munter T. 5, Manoli K. 2, Palazzo G. 2, Torsi L. 2
Published in: Analytical and Bioanalytical Chemistry, 2016, Vol. 408(15), p. 3943-52
In this contribution, we propose a label-free immunosensor, based on a novel type of electrolyte-gated field-effect transistor (EGOFET), for ultrasensitive detection of the C-reactive protein (CRP). [...]

Amine-appended polyaniline as a water dispersible electroactive polyelectrolyte and its integration into functional self-assembled multilayers

Publication year: 2016
Authors: Waldemar A. Marmisollé a, Eliana Mazaa b, Sergio Moya b, Omar Azzaroni a
Published in: Electrochimica Acta, 2016, Vol. 210, p. 435–444
We present an effective and simple method for the synthesis of poly(3-aminobenzylamine) (PABA) using a chemical oxidation strategy in aqueous solution. The polymer was characterized by NMR, LDI-TOF mass spectrometry, UV-visible, XPS and ATR-FTIR spectroscopies. [...]

Kinetic studies of bovine serum albumin interaction with PG and TBHQ using surface plasmon resonance

Publication year: 2016
Authors: Fathi F. a,b,c, Ezzati Nazhad Dolatanbadi J. a, Rashidi M.R. a, Omidi Y. a
Published in: International Journal of Biological Macromolecules, 2016, Vol. 91, p. 1045–1050
Propyl gallate (PG) and tert-butylhydroquinone (TBHQ) are examples of phenolic antioxidant agents, which have widespread uses in food industry. In this study, for the first time, we report on the interaction of PG and TBHQ [...]

Layer-by-layer assembly of functionalized reduced graphene oxide for direct electrochemistry and glucose detection

Publication year: 2016
Authors: Mascagni D.B. a, Miyazaki C.M. b, da Cruz N.C. a, de Moraes M.L. c, Riul A. Jr. d, Ferreira M. b
Published in: Materials Science and Engineering: C, 2016, Vol. 68, p. 739–745
We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. [...]

Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk

Publication year: 2016
Authors: Farka Z. 1, Juřík T. 1, 2, Pastucha M. 1, 2, Skládal P.1, 2
Published in: Analytical Chemistry, 2016, Vol. 88 (23), p. 11830–11836
Contamination of food by pathogenic bacteria has always been a serious threat for human health. The amount of food exports and imports has been increasing in recent years which requires precise food quality control [...]

SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System

Publication year: 2016
Authors: Millone M.A.D. 1, Ramirez E.A. 1, Chain C.Y. 1, Crivaro A. 2, Romanin D. 2, Rumbo M. 2, Docena G. 2, Cocco M.D. 3, Pedano M.L. 3, Fainstein A. 3, Montoya J. 4, Vela M.E. 1, Salvarezza R.C. 1
Published in: Journal of Nanomaterials, Vol. 2016, Article ID 5432656, 9 p.
Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins. These substances typically have low molecular weight, [...]

Incorporation of gold nanoparticles into Langmuir-Blodgett films of polyaniline and montmorillonite for enhanced detection of metallic ions

Publication year: 2016
Authors: A. De Barros a, b, C.J.L. Constantino c, J.R.R. Bortoleto d, N.C. Da Cruz d, M. Ferreira b
Published in: Sensors and Actuators B: Chemical, Vol. 236, 2016, p. 408–417
We report on effects from incorporating gold nanoparticles (AuNPs) into Langmuir-Blodgett (LB) films of emeraldine salt polyaniline (PAni-ES) and organophilic montmorillonite clay (OMt). [...]

HRP-Mimicking DNAzyme-Catalyzed in Situ Generation of Polyaniline To Assist Signal Amplification for Ultrasensitive Surface Plasmon Resonance Biosensing

Publication year: 2016
Authors: Haiyin Li, Jiafu Chang, Ting Hou, Feng Li
Published in: Analytical Chemistry, 2017, Vol. 89 (1), p. 673–680
It is well-known that the horseradish peroxidase- (HRP-) mimicking DNAzyme, namely, hemin/G-quadruplex, can effectively catalyze the polymerization of aniline to form DNA-guided polyaniline. [...]

SPR analysis of the interaction between a recombinant protein of unknown function in Leishmania infantum immobilised on dendrimers and antibodies of the visceral leishmaniasis: A potential use in immunodiagnosis

Publication year: 2015
Authors: Souto D.E. 1, Fonseca A.M. 2, Barragan J.T. 1, Luz Rde C. 3, Andrade H.M. 2, Damos F.S. 3, Kubota L.T. 4
Published in: Biosensors and Bioelectronics, 2015, Vol. 70, p. 275-81
In this work, an SPR immunosensor was developed to elucidate the reaction kinetics between a protein of unknown function in Leishmania infantum (hypothetical C1 protein) and specific antibodies of [...]

UV crosslinked poly(acrylic acid): a simple method to bio-functionalize electrolyte-gated OFET biosensors

Publication year: 2015
Authors: M. Y. Mulla a, P. Seshadri a, L. Torsi a, K. Manoli a, A. Mallardi b, N. Ditaranto a, M. V. Santacroce c, C. Di Franco c, G. Scamarcio c and M. Magliulo, *a
Published in: Journal of Materials Chemistry B, 2015, Vol. 3, p. 5049-5057
A simple and time-saving wet method to endow the surface of organic semiconductor films with carboxyl functional groups is presented. A thin layer of poly(acrylic acid) (pAA) is spin-coated directly on the electronic channel of [...]

Surface Dynamics Study of DNA Loop Formation Using Surface Plasmon Resonance

Publication year: 2015
Authors: Mary Arugula, Fuling Yang, Subramaniam Somasundaram, Christopher J Easley, Curtis Shannon and Aleksandr Simonian
Published in: ECS Transactions, 2015, Vol. 66, Issue 36, p. 19-29
Electrochemical proximity assay (ECPA), was recently developed for direct protein quantitation as low as 158 fM. In this assay, the loop-like DNA structure holds methylene blue (MB-DNA) in close proximity with the gold surface when target is present, ensuring efficient electron transfer. However, for studying DNA binding [...]

Biosensors for Detecting Genetically Modified Organisms in Food and Feed

Publication year: 2015
Authors: Mary Arugula, Alina Chanysheva, Kiril Vaglenov and Aleksandr Simonian
Published in: ECS Transactions, 2015, Vol. 66, Issue 36, p. 31-38
The detection of genetically modified organisms is of growing interest given their considerable presence in vast variety of food and feed ingredients, as well as their environmental impact. In this study we demonstrate real-time, label- free detection of DNA from genetically modified, Roundup Ready (RR) soybeans by surface plasmon resonance (SPR). [...]

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces

Publication year: 2015
Authors: Zhou L. a, Arugula M.A. a, Easley C. b, Shannon C. b, Simonian A.L. a
Published in: ECS Transactions, 2015, Vol. 69 (38), p. 9-16
With increasing significance of characterization in functional interfaces, a Multi-Parametric/Multimodal Spectroscopy Apparatus was proposed here to enhance the power of interface characterization sensing techniques. [...]

Using QCM and SPR for the Kinetic Evaluation of the Binding Between A New Recombinant Chimeric Protein and Specific Antibodies of the Visceral Leishmaniasis

Publication year: 2015
Authors: Souto D.E., Faria A.R., de Andrade H.M., Kubota L.T.
Published in: Current Protein and Peptide Science, 2015, Vol. 16, p. 782-790
In the present study, the surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) techniques were employed to kinetically evaluate the binding affinity of a new recombinant chimeric protein (CP10) toward anti-Leishmania infantum antibodies [...]

Synergy between Polyaniline and OMt Clay Mineral in Langmuir–Blodgett Films for the Simultaneous Detection of Traces of Metal Ions

Publication year: 2015
Authors: de Barros A. 1,2, Ferreira M. 3, Constantino C.J.L. 4, Bortoleto J.R.R. 5, Ferreira M. 2
Published in: ACS, Applied Materials and Interfaces, 2015, Vol. 7 [12], p. 6828–6834
We report on Langmuir–Blodgett (LB) films made with emeraldine salt polyaniline (PAni-ES) and organophilic montmorillonite clay mineral (OMt), where synergy between the components was reached to yield an enhanced performance in detecting trace levels of cadmium (Cd2+), lead (Pb2+) and copper (Cu2+). [...]

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Publication year: 2015
Authors: Miyazaki C.M. 1, Pereira T.P. 1, Tavares Mascagni D.B. 2, de Moraes M.L. 3, Ferreira M. 1
Published in: Materials Science and Engineering: C, 2015, Vol. 58, p. 310–315
In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. [...]