Publications: Biosensors

to

Detection of transferrin by using a surface plasmon resonance sensor functionalized with a boronic acid monolayer

Publication year: 2017
Authors: Yanchao Mayang 1,2, Xiwen He 1,2, Langxing Chen 1,2, Yukui Zhang 1,2,3
Published in: Microchimica Acta, 2017, Vol. 184 (8), p. 2749–2757
A surface plasmon resonance sensor whose surface has been modified with boronic acid groups was developed in order to bind and detect transferrin. 4-Mercaptophenylboronic acid (4-MPBA) was utilized [...]

Integration of Biorecognition Elements on PEDOT Platforms through Supramolecular Interactions

Publication year: 2017
Authors: Sappia L.D. 1,2, Piccinini E. 3, Marmisollé W. 3, Santilli N. 1, Maza E. 4, Moya S. 4, Battaglini F. 5, Madrid R.E. 1,2, Azzaroni O. 3
Published in: Advanced Materials Interfaces, 2017, 1700502
The rapidly emerging field of organic bioelectronics exploits the functional versatility of conducting polymers to transduce biological recognition events into electronic signals. For the majority of biosensors [...]

Investigation of graphene-on-metal substrates for SPR-based sensor using finite-difference time domain

Publication year: 2017
Authors: Said F. A. 1, Menon P. S. 1, Rajendran V. 2, Shaari S. 1, Majlis B. Y. 1
Published in: IET Nanobiotechnology, 2017, Vol. 11, Issue 8, p. 981 – 986
In this study, the authors investigated the effects of a single layer graphene as a coating layer on top of metal thin films such as silver, gold, aluminum and copper using finite-difference time domain method. [...]

Immuno-biosensor for Detection of CD20-Positive Cells Using Surface Plasmon Resonance

Publication year: 2017
Authors: Shanehbandi D. 1,2, Majidi J. 1, Kazemi T. 1,2, Baradaran B. 1, Aghebati-Maleki L. 1,2, Fathi F. 2,3, Ezzati Nazhad Dolatabadi J. 3
Published in: Advanced Pharmaceutical Bulletin, 2017, Vol. 7 (2), p.188-194
Purpose: Surface plasmon resonance (SPR) sensing confers a real-time assessment of molecular interactions between biomolecules and their ligands. This approach is highly sensitive [...]

Characterization of IFN-γ Detection and Electrochemical Proximity Assay by a Novel Multi-Parametric/Multimodal Spectroscopy Apparatus

Publication year: 2017
Authors: Zhou L., Chin B. A., Simonian A. L.*
Published in: ECS Transactions, 2017, Vol. 80, Issue 10, p. 1565-1570
Functional interfaces play a vital role in biosensing, which requires proper allocation, orientation, conformation, activities and stability to achieve high affinity and stability of the biomolecules. However, the characterization [...]

Characterization of Covalently Bound Anti-Human Immunoglobulins on Self-Assembled Monolayer Modified Gold Electrodes

Publication year: 2017
Authors: Holzer B. 1, Manoli K. 1, Ditaranto N. 1,2, Macchia E. 1, Tiwari A. 1, Di Franco C. 3, Scamarcio G. 3,4, Palazzo G. 1,2, Torsi L. 1,2
Published in: Advanced Biosystems, 2017
Bioconjugated gold surfaces constitute interesting platforms for biosensing applications. The immobilization of antibodies such as anti-immunoglobulin G and M (anti-IgG and anti-IgM) [...]

Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk

Publication year: 2016
Authors: Farka Z. 1, Juřík T. 1, 2, Pastucha M. 1, 2, Skládal P.1, 2
Published in: Analytical Chemistry, 2016, Vol. 88 (23), p. 11830–11836
Contamination of food by pathogenic bacteria has always been a serious threat for human health. The amount of food exports and imports has been increasing in recent years which requires precise food quality control [...]

SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System

Publication year: 2016
Authors: Millone M.A.D. 1, Ramirez E.A. 1, Chain C.Y. 1, Crivaro A. 2, Romanin D. 2, Rumbo M. 2, Docena G. 2, Cocco M.D. 3, Pedano M.L. 3, Fainstein A. 3, Montoya J. 4, Vela M.E. 1, Salvarezza R.C. 1
Published in: Journal of Nanomaterials, Vol. 2016, Article ID 5432656, 9 p.
Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins. These substances typically have low molecular weight, [...]

Incorporation of gold nanoparticles into Langmuir-Blodgett films of polyaniline and montmorillonite for enhanced detection of metallic ions

Publication year: 2016
Authors: A. De Barros a, b, C.J.L. Constantino c, J.R.R. Bortoleto d, N.C. Da Cruz d, M. Ferreira b
Published in: Sensors and Actuators B: Chemical, Vol. 236, 2016, p. 408–417
We report on effects from incorporating gold nanoparticles (AuNPs) into Langmuir-Blodgett (LB) films of emeraldine salt polyaniline (PAni-ES) and organophilic montmorillonite clay (OMt). [...]

HRP-Mimicking DNAzyme-Catalyzed in Situ Generation of Polyaniline To Assist Signal Amplification for Ultrasensitive Surface Plasmon Resonance Biosensing

Publication year: 2016
Authors: Haiyin Li, Jiafu Chang, Ting Hou, Feng Li
Published in: Analytical Chemistry, 2017, Vol. 89 (1), p. 673–680
It is well-known that the horseradish peroxidase- (HRP-) mimicking DNAzyme, namely, hemin/G-quadruplex, can effectively catalyze the polymerization of aniline to form DNA-guided polyaniline. [...]

Adsorption Studies of Organophosphonic Acids on Differently Activated Gold Surfaces

Publication year: 2016
Authors: Katrin Niegelhell †, Simon Leimgruber †‡, Thomas Grießer §, Christian Brandl ∥, Boril Chernev ∥, Robert Schennach ⊥, Gregor Trimmel †, and Stefan Spirk †
Published in: Langmuir, 2016, Vol. 32 (6), p. 1550–1559
In this study, the formation of self-assembled monolayers consisting of three organophosphonic acids (vinyl-, octyl-, and tetradecylphosphonic acid) from isopropanol solutions onto differently activated gold surfaces is studied in situ and in real time using multiparameter surface plasmon resonance (MP-SPR). [...]

Label-free C-reactive protein electronic detection with an electrolyte-gated organic field-effect transistor-based immunosensor

Publication year: 2016
Authors: Magliulo M. 1, De Tullio D. 2, Vikholm-Lundin I. 3, Albers W.M. 4, Munter T. 5, Manoli K. 2, Palazzo G. 2, Torsi L. 2
Published in: Analytical and Bioanalytical Chemistry, 2016, Vol. 408(15), p. 3943-52
In this contribution, we propose a label-free immunosensor, based on a novel type of electrolyte-gated field-effect transistor (EGOFET), for ultrasensitive detection of the C-reactive protein (CRP). [...]

Amine-appended polyaniline as a water dispersible electroactive polyelectrolyte and its integration into functional self-assembled multilayers

Publication year: 2016
Authors: Waldemar A. Marmisollé a, Eliana Mazaa b, Sergio Moya b, Omar Azzaroni a
Published in: Electrochimica Acta, 2016, Vol. 210, p. 435–444
We present an effective and simple method for the synthesis of poly(3-aminobenzylamine) (PABA) using a chemical oxidation strategy in aqueous solution. The polymer was characterized by NMR, LDI-TOF mass spectrometry, UV-visible, XPS and ATR-FTIR spectroscopies. [...]

Kinetic studies of bovine serum albumin interaction with PG and TBHQ using surface plasmon resonance

Publication year: 2016
Authors: Fathi F. a,b,c, Ezzati Nazhad Dolatanbadi J. a, Rashidi M.R. a, Omidi Y. a
Published in: International Journal of Biological Macromolecules, 2016, Vol. 91, p. 1045–1050
Propyl gallate (PG) and tert-butylhydroquinone (TBHQ) are examples of phenolic antioxidant agents, which have widespread uses in food industry. In this study, for the first time, we report on the interaction of PG and TBHQ [...]

Layer-by-layer assembly of functionalized reduced graphene oxide for direct electrochemistry and glucose detection

Publication year: 2016
Authors: Mascagni D.B. a, Miyazaki C.M. b, da Cruz N.C. a, de Moraes M.L. c, Riul A. Jr. d, Ferreira M. b
Published in: Materials Science and Engineering: C, 2016, Vol. 68, p. 739–745
We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. [...]

Using QCM and SPR for the Kinetic Evaluation of the Binding Between A New Recombinant Chimeric Protein and Specific Antibodies of the Visceral Leishmaniasis

Publication year: 2015
Authors: Souto D.E., Faria A.R., de Andrade H.M., Kubota L.T.
Published in: Current Protein and Peptide Science, 2015, Vol. 16, p. 782-790
In the present study, the surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) techniques were employed to kinetically evaluate the binding affinity of a new recombinant chimeric protein (CP10) toward anti-Leishmania infantum antibodies [...]

Synergy between Polyaniline and OMt Clay Mineral in Langmuir–Blodgett Films for the Simultaneous Detection of Traces of Metal Ions

Publication year: 2015
Authors: de Barros A. 1,2, Ferreira M. 3, Constantino C.J.L. 4, Bortoleto J.R.R. 5, Ferreira M. 2
Published in: ACS, Applied Materials and Interfaces, 2015, Vol. 7 [12], p. 6828–6834
We report on Langmuir–Blodgett (LB) films made with emeraldine salt polyaniline (PAni-ES) and organophilic montmorillonite clay mineral (OMt), where synergy between the components was reached to yield an enhanced performance in detecting trace levels of cadmium (Cd2+), lead (Pb2+) and copper (Cu2+). [...]

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Publication year: 2015
Authors: Miyazaki C.M. 1, Pereira T.P. 1, Tavares Mascagni D.B. 2, de Moraes M.L. 3, Ferreira M. 1
Published in: Materials Science and Engineering: C, 2015, Vol. 58, p. 310–315
In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. [...]

Recognition-driven assembly of self-limiting supramolecular protein nanoparticles displaying enzymatic activity

Publication year: 2015
Authors: Piccinini E. a, Pallarola D. a, Battaglini F. b, Azzaroni O. *a
Published in: Chemical Communications, 2015, Issue 79, p. 14754-7
We report the recognition-driven assembly of self-limiting protein nanoparticles displaying enzymatic activity. Solution self-assembly of concanavalin A lectin and glycoenzyme glucose oxidase leads to [...]

SPR analysis of the interaction between a recombinant protein of unknown function in Leishmania infantum immobilised on dendrimers and antibodies of the visceral leishmaniasis: A potential use in immunodiagnosis

Publication year: 2015
Authors: Souto D.E. 1, Fonseca A.M. 2, Barragan J.T. 1, Luz Rde C. 3, Andrade H.M. 2, Damos F.S. 3, Kubota L.T. 4
Published in: Biosensors and Bioelectronics, 2015, Vol. 70, p. 275-81
In this work, an SPR immunosensor was developed to elucidate the reaction kinetics between a protein of unknown function in Leishmania infantum (hypothetical C1 protein) and specific antibodies of [...]