Label-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules

Publication year: 2019
Authors: Tasbasi BB 1, Guner BC 1, Sudagidan M 1, Ucak S 2, Kavruk M3, Ozalp VC 4.
Affiliations:

1 Kit-Argem, Konya Food and Agriculture University, 42080, Konya, Turkey.

2 Altınbas University, School of Medicine, Department of Medical Biology, 34217, Istanbul, Turkey.

3 Gebze Quality Campus, Turkish Standards Institution (TSE), 41400, Gebze, Kocaeli, Turkey.

4 Kit-Argem, Konya Food and Agriculture University, 42080, Konya, Turkey. 

Published in: Analytical Biochemistry
doi: DOI: 10.1016/j.ab.2019.113449

Lateral flow assay (LFA) type of biosensors have been popular due to cost-effectiveness and easy-interpretation for instant results, most common examples of applications being pregnancy tests, food safety or medical diagnostics. There are several examples of reports with high sensitivity, including pre-concentration of the sample by magnetic pull-down. However, sensitivity and direct detection designs with aptamers has been a limiting factor for developing aptamers-based LFA assays. In this study, we report a lateral flow design based on aptamer-gated silica nanoparticles to develop high sensitivity and direct bacterial assay by shifting aptamers-target interaction to conjugation pad. Aptamer-gated silica nanoparticles-based biosensors were reported for their high sensitivity, specificity and label-free detection for small molecules and whole cells. This label-free strategy for LFA can determine L. monocytogenes in minced chicken matrix at less than 5 min with a limit of detection (LOD) of 53 cells in one mL samples.


MP-SPR keywords: aptamer, bacteria detection, biosensor development, streptavidin