Trypanosoma cruzi Virulence Factors for the Diagnosis of Chagas’ Disease

Publication year: 2019
Authors: Cecilia Yamil Chain,*,† Dênio Emanuel Pires Souto,*,‡ María Laura Sbaraglini,§ Carlos A. Labriola,∥ María Antonieta Daza Millone,† Eduardo Alejandro Ramirez,† José Sebastián Cisneros,† Constanza Lopez-Albizu,⊥ Karenina Scollo,⊥ Lauro T. Kubota,# Andrés Mariano Ruiz,⊥ and María Elena Vela†
Affiliations:

†Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Universidad Nacional de La Plata-CONICET, Diagonal 113 y 64 S/N, La Plata 1900, Argentina ‡Department of Chemistry, Federal University of Paraná(UFPR), Avenida Coronel Francisco Heráclito dos Santos, 100; Jardim das Américas, Curitiba, Paraná 81530-000, Brazil §Laboratorio de Investigación y Desarrollo de Bioactivos (LIDeB), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, calle 47 200-236, La Plata ARB1900AJJ, Argentina ∥Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA-CONICET), Universidad Nacional de Buenos Aires, Av. Patricias Argentinas 435, Ciudad de Buenos Aires AR C1405BWE, Argentina ⊥Instituto Nacional de Parasitología “Dr. Mario Fatala Chabén”-Administración Nacional de Institutos y Laboratorios de Salud (ANLIS), Avenida Paseo Colón 568, Ciudad Autónoma de Buenos Aires C1063ACR, Argentina #Instituto Nacional de Ciência e Tecnologia em Bioanalítica, Unicamp, Rua Josué de Castro s/n°, Cidade Universitária-Barão Geraldo, Campinas, São Paulo 13083-861, Brazil

Published in: ACS Infectious Diseases
doi: DOI:10.1021/acsinfecdis.9b00269

trans-Sialidase and cruzipain are important virulence factors from Trypanosoma cruzi, the etiological agent of Chagas disease, that have highly antigenic domains in their structure and were reported as potential tools for diagnosis of the illness. The aim of the present study is to assess the possibility of using cruzipain and the catalytic domain of trans-sialidase in a Surface Plasmon Resonance-based immunosensor for the diagnosis of chronic Chagas disease. Immunoassays carried out with canine sera verified that cruzipain allows the detection of anti-Trypanosoma cruzi antibodies whereas recombinant trans-sialidase did not yield specific detections, due to the high dilutions of serum used in the immunoassays that hinder the possibility to sense the specific low titer antibodies. The developed cruzipain-based biosensor, whose price per assay is comparable to a commercial enzyme-linked immunosorbent assay (ELISA), was successfully applied for the rapid quantification of specific antibodies against Trypanosoma cruzi in fresh human sera showing an excellent agreement with ELISA. 


MP-SPR keywords: Au sensor slide, Chaga’s Disease, diagnostics, immunosensor, SAM, serum, virulence factor