Nanoparticle uptake by cells measured using MP-SPR

Application Note #156


Figure 3.
Uptake of mesoporous silica nanoparticles in human epithelial cervical cancer cells (HeLa). Positively charged nanoparticles (C-SiNPs) showed a more efficient uptake and caused a larger response in MP-SPR than negatively charged NPs (P-SiNPs). There was a rapid initial response when SiNPs reached the flow-cell, due to some remaining stock solvent dimethyl sulfoxide (DMSO) after dilutions.

Nanoparticles (NPs) are extensively studied as drug delivery systems. NPs enter the cells usually by active transport, i.e. endocytosis. Multi-Parametric Surface Plasmon Resonance (MP-SPR) is previously used for protein-protein interactions is now used in pioneering NP – living cell studies. Confluent monolayers of human epithelial cervical cancer cells (HeLa) were grown on sensor slides. Uptake of mesoporous silica nanoparticles (SiNPs), branched polyethyleneimine–DNA polyplexes (bPEI–DNA PPs), and extracellular vesicles (EVs) were studied using MP-SPR. Uptake was measured at different temperatures and the activation energy of the cell uptake was calculated using Arrhenius plots.

Recommended instrument for this application:

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Further reading

  • If you are interested in drug delivery nanoparticles, we recommend to have a look at AN#152 interactions of drug delivery nanoparticles and AN#151 about measurement of soft and hard corona on nanoparticle in 100% serum. If you are interested in living cell studies, see AN#137 about small molecules interaction with cell monolayer and AN#154 about cell adhesion on surface and cancer cell detection.

  • Have a look at original publication here: Suutari et al. (2016)

  • Do you want to see how MP-SPR instruments work?  Click here.

  • Do you want to see comparison of MP-SPR instruments? Click here.

 

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