MP-SPR measurements of soft and hard corona on nanoparticle in 100% serum

Application Note #151

Figure 2. Sensogram during serum interaction on the DOX liposomes (replicas of Doxil© liposomes without doxorubicin). Based on layer thickness and refractive index information, a thinner soft corona was formed on PEGylated liposomes, whereas hard corona was thicker when compared to liposomes without PEGylation.

When nanocarriers are introduced into the blood circulation, they are rapidly covered with a protein corona, which is a complex layer of biomolecules such as proteins, lipids, and other plasma components. Further cellular responses to the nanoparticle depend on the composition of corona.

Interaction of lipid based nanocarriers with 100% serum was studied using Multi-Parametric Surface Plasmon Resonance (MP-SPR). Protein corona formation on a nanocarrier surface was studied without disturbing its dynamics. Positively charged liposomes with oligo guanidyl lipid derivative (OGD) and replicas of the Doxil© without doxorubicin (DOX) were studied with and without PEGylation. Thickness and refractive index of lipid and corona layers were determined. The thickness of soft corona on OGD liposomes was 34.2 nm, whereas hard corona thickness was 9.6 nm.

Exploiting PureKinetics™ feature makes MP-SPR measurements suitable even in 100% serum samples but also for measurements in other complex liquids such as saliva (Sonny 2010), milk (Ye 2016) or sea water (Ma 2015).

Recommended instrument for this application:

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Further reading

  • If you are interested in drug delivery, we recommend to have a look at AN#152 about interactions of drug delivery nanocarriers and AN#156 about uptake of nanoparticles by living cells. If you are interested in molecular interactions, you may view AN#155 about faster interaction measurements using KineticTitration.
  • Do you want to learn more about PureKinetics™? Click here.

  • Do you want to see how MP-SPR instruments work? Click here.

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