Small molecular weight drug-protein interaction measured with MP-SPR

Application Note #144

 
''Kinetic evaluation of indomethacin binding. Different concentrations of analyte (coloured curves) and fit (black curves).''

The interaction of a small molecular weight drug (indomethacin) to Human serum albumin (HSA) was measured with label-free Multi-Parametric Surface Plasmon Resonance (MP-SPR). HSA was attached to a sensor surface with amine coupling chemistry. Affinity (KD) and kinetic constants (ka and kd) of indomethacin interaction were determined with TraceDrawer™ for MP-SPR Navi™. Premium quality kinetic data was ensured using PureKinetics™ feature that separates bulk effect from binding kinetics.

Recommended instrument for this application:

200_otso_small.jpg 210_vasa_small.jpg 220A_naali_small.jpg
420_ilves_small.jpg

    green-tick.png green-tick.png 

Further reading

  • If you are interested in reading more about small molecules, have a look at AN#155 about faster interaction measurements using KineticTitration and AN#137 about cell interactions.

  • Do you want to find out how to calculate thickness out of MP-SPR measurements? Click here.

  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see MP-SPR instruments comparison? Click here.

  • Here are a few publications you can have a look at.

DOWNLOAD APPLICATION NOTE IN PDF


Back to all Application Notes