Real-time monitoring of antibody binding by parallel MP-SPR, potentiometry and impedance spectroscopy

Application Note #143


Figure 1
. MP-SPR measurement combined with potentiostat with BioNavis electrochemistry flow-cell.

Multi-Parametric Surface Plasmon Resonance (MP-SPR) equipped with an electrochemistry (EC) flow-cell was used to run potentiometric and impedance measurements in parallel with the MP-SPR measurement. The binding reaction of anti-methotrexate to immobilized small molecule weight drug methotrexate (MTX) was followed by MP-SPR and by potentiometry and Electrochemical Impedance Spectroscopy (EIS). Methotrexate binding produced a relatively large impedance response and antibody molecules a small response, whereas potentiometry values stayed equal during interactions. The strong binding of antibody molecules was observed in the MP-SPR response.

Recommended instrument for this application:

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Further reading

  • If you are interested in reading more, see AN#125 on how to study the binding kinetics of single chain antibodies of certain specificities by MP-SPR or AN#142 about metal stripping and deposition using EC-MP-SPR.

  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see MP-SPR instruments comparison? Click here.

  • Here are a few publications you can have a look at.

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