Supported lipid layers characterization with MP-SPR

Application Note #139


"SPR sensograms from lipid bilayers and vesicular layers deposition on different substrates"

Supported lipid membranes can be used in vitro experiments to mimic biological barriers, such as cell membranes. It is also possible to incorporate other molecules, like receptors or membrane proteins into the lipid layer structure to mimic specific functionality of the biological membrane. Due to these properties supported lipid layer structures can be widely utilized in field of biochemistry and pharmaceutical research.

MP-SPR was utilized to characterize lipid layer structures on SiO2, 6kDa dextran and thiolated polyethylene glycol support. Thickness and refractive index of deposited lipid layers was determined. Bilayers thickness was 5nm whereas vesicular layer thickness was 10nm.

Recommended instrument for this application:

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Further reading

  • If you are interested in lipids, we recommend to have a look at AN#157 about detection of toxin induced pore formation in membranes and AN#151 about measurement of soft and hard corona on liposomes.

  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see comparison of MP-SPR instruments? Click here.

  • Here are a few publications you can have a look at.


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