SPR Navi™ Immobilizer

Application Note #130

Langmuir-blodgett


SPR Navi™ Immobilizer allows ex situ immobilization at wide range of temperatures, while maintaining low reagent consumption and saving instrument time. The stand-alone immobilizer was used to prepare dNA-thiol SAM with N,N-bis (2-hydroxyethyl)-α-lipoamide to avoid non-specific binding during experiment.

SPR Navi™ Immobilizer is a small stand-alone device for functionalization of sensor slides used in SPR Navi™ Multi Parametric Surface Plasmon Resonance (MP–SPR) instruments.

Compatibility. SPR Navi™ Immobilizer is compatible with the SPR Navi™ sensor slides and SPR Navi™ sensor slide holder. The immobilizer forms two channels, which copy the shape of the SPR Navi™ standard flow-cell (Fig. 1). Liquid reagents can be inserted in the chambers with a syringe or a pump via tubing connection.

Extended immobilization temperature. The SPR Navi™ Immobilizer is portable and thus, can be also placed on a hot plate or in an incubator, for instance. Immobilization on a hot plate enables reactions at higher or lower temperatures than those possible in situ using SPR Navi™ instruments.

Low reagent consumption. The immobilizer enables ex situ immobilization with minimized reagent consumption. After immobilization, only the channel area is covered with the reagents. While ex situ immobilization on a Petri-dish requires covering the whole sensor slide area (240 mm2), the ex situ immobilization with SPR Navi™ Immobilizer requires covering of channel area only (24 mm2). The total internal volume of the device is a few tens of microliters, allowing small volumes to be used.

Save instrument time. The ex situ sensor preparation with an external SPR Navi™ Immobilizer can save instrument time from immobilization to actual measurements. An especially useful property, when the SPR Navi™ instrument is in heavy use.

Recommended instrument for this application:

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Further reading

  • If you are interested in reading more about dna treatment and DNA assay, please view AN#117.

  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see comparison of MP-SPR instruments? Click here.
  • Here are a few publications you can have a look at.

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