Protein interaction with cellulose studied with MP-SPR
and QCM-D

Application Note #126


"Selecting surface modification for in vitro diagnostic tests"
Adsorption of hIgG on cellulose, chitosan and CMC surfaces at pH 5-8 studied with MP-SPR.

Nanocellulose is a promising new type of material for point-of-care diagnostic devices and other low cost consumer analysis devices. The prevention of non-specific adsorption of biomolecules is a key to detection sensitivity of the system and evaluation of its suitability for give application. Hence, a BSA adsorption study was performed. Detection of certain pathogen requires specific affinities. Functionalization of cellulose substrates with hIgG can be a viable platform for biopolymer detection. 

The adsorption of human immunoglobulin G (hIgG) and bovine serum albumin (BSA) on native-, carboxymethylated cellulose (CMC) -modified and chitosan-modified cellulose surface was studied with MP-SPR (BioNavis, SPR Navi™ 200) and QCM-D (Q Sense, E4). 

The results show that it is possible to tailor biomolecule binding by choice of appropriate surface modifier and pH of the medium. 

Recommended instrument for this application:

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Further reading

  • If you are interested in cellulose, you may also view AN#149 about functionalization of nanocellulose, AN#159 about swelling of nanocrystalline cellulose in water vapor and AN#158 about predicting cellulose nanocrystals dispersibility.
  • Do you want to see MP-SPR instruments comparison? Click here.
  • If you are interested in reading more about nanocellulose, we recommend to have a look our publications.


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