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Recognition-driven assembly of self-limiting supramolecular protein nanoparticles displaying enzymatic activity

Publication year: 2015
Authors: Piccinini E. a, Pallarola D. a, Battaglini F. b, Azzaroni O. *a
Published in: Chemical Communications, 2015, Issue 79, p. 14754-7
We report the recognition-driven assembly of self-limiting protein nanoparticles displaying enzymatic activity. Solution self-assembly of concanavalin A lectin and glycoenzyme glucose oxidase leads to [...]

Adsorption and lubricating properties of HFBII hydrophobins and diblock copolymer poly(methyl methacrylate-b-sodium acrylate) additives in water-lubricated copper vs. a-C:H contacts

Publication year: 2015
Authors: Hakala TJ1, Metsäjoki J1, Granqvist N2, Milani R1, Szilvay GR1, Elomaa O3, Deng M4, Zhang J4, Li F3
Published in: Tribology International, Volume 90, October 2015, Pages 60–66
In a metal forming process the adhesion between the workpiece and the tool needs to be minimised, which can be achieved by use of lubricants and coatings. [...]

Synergy between Polyaniline and OMt Clay Mineral in Langmuir–Blodgett Films for the Simultaneous Detection of Traces of Metal Ions

Publication year: 2015
Authors: de Barros A. 1,2, Ferreira M. 3, Constantino C.J.L. 4, Bortoleto J.R.R. 5, Ferreira M. 2
Published in: ACS, Applied Materials and Interfaces, 2015, Vol. 7 [12], p. 6828–6834
We report on Langmuir–Blodgett (LB) films made with emeraldine salt polyaniline (PAni-ES) and organophilic montmorillonite clay mineral (OMt), where synergy between the components was reached to yield an enhanced performance in detecting trace levels of cadmium (Cd2+), lead (Pb2+) and copper (Cu2+). [...]

Chelating Surfaces for Native State Proteins Patterning: The Human Serum Albumin Case

Publication year: 2015
Authors: Giamblanco N., Tuccitto N., Zappalà G., Sfuncia G., Licciardello A., Marletta G.
Published in: ACS Applied Materials & Interfaces, 2015, Vol. 7 (41), p. 23353–23363
The paper reports a new “soft” surface functionalization strategy, based on a highly selective ion metal chelation process. The proposed stepwise methodology implies at first the construction of a monolayer of terpyridine-based thiol (Tpy), whose highly packed [...]

Interactions between Cellulolytic Enzymes with Native, Autohydrolysis, and Technical Lignins and the Effect of a Polysorbate Amphiphile in Reducing Nonproductive Binding

Publication year: 2015
Authors: Fritz C. 1, Ferrer A. 1, Salas C. 1, Jameel H. 1, Rojas O.J. 1,2
Published in: Biomacromolecules, 2015, Vol. 16(12), p. 3878-88
Understanding enzyme-substrate interactions is critical in designing strategies for bioconversion of lignocellulosic biomass. In this study we monitored molecular events, in situ and in real time, including the adsorption and desorption of cellulolytic enzymes on lignins and cellulose, by using quartz crystal microgravimetry and surface plasmon resonance. [...]

Cholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes

Publication year: 2015
Authors: Alm I., García-Linares S., Gavilanes J.G., Martínez-Del-Pozo Á., Slotte J.P.
Published in: Biochimica et Biophysica Acta, 2015, Vol. 1848(4), p. 925-31
The pore forming capacity of Sticholysin II (StnII; isolated from Stichodactyla helianthus) in bilayer membranes containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), palmitoylsphingomyelin (PSM) and either cholesterol or palmitoyl ceramide (PCer) has been examined. The aim of the study was to elucidate how the presence of differently ordered PSM domains affected StnII oligomerization and pore formation. [...]

UV crosslinked poly(acrylic acid): a simple method to bio-functionalize electrolyte-gated OFET biosensors

Publication year: 2015
Authors: M. Y. Mulla a, P. Seshadri a, L. Torsi a, K. Manoli a, A. Mallardi b, N. Ditaranto a, M. V. Santacroce c, C. Di Franco c, G. Scamarcio c and M. Magliulo, *a
Published in: Journal of Materials Chemistry B, 2015, Vol. 3, p. 5049-5057
A simple and time-saving wet method to endow the surface of organic semiconductor films with carboxyl functional groups is presented. A thin layer of poly(acrylic acid) (pAA) is spin-coated directly on the electronic channel of [...]

Investigation of pH-Induced Protein Conformation Changes by Nanomechanical Deflection

Publication year: 2014
Authors: Thakur G. †, Jiang K. †, Lee D. †, Prashanthi K. †, Kim S. †‡, Thundat T. †
Published in: Langmuir, 2014, Vol. 30 (8), p. 2109–2116
Broad-spectrum biosensing technologies examine sensor signals using biomarkers, such as proteins, DNA, antibodies, specific cells, and macromolecules, based on direct- or indirect-conformational changes. [...]

Grafting hyaluronic acid onto gold surface to achieve low protein fouling in surface plasmon resonance biosensors

Publication year: 2014
Authors: Liu X., Huang R., Su R., Qi W., Wang L., He Z.
Published in: ACS Applied Materials and Interfaces, 2014, Vol. 6(15), p. 13034-42
Antifouling surfaces capable of reducing nonspecific protein adsorption from natural complex media are highly desirable in surface plasmon resonance (SPR) biosensors. A new protein-resistant surface [...]

Adsorption behavior of tightly bound extracellular polymeric substances on model organic surfaces under different pH and cations with surface plasmon resonance

Publication year: 2014
Authors: Zhang P. 1, Chen Y.P. 2, Guo J.S. 3, Shen Y. 4, Yang J.X. 4, Fang F. 1, Li C. 1, Gao X. 1, Wang G.X. 5
Published in: Water Research, 2014, Vol.57, p. 31-9
Tightly bound extracellular polymeric substances (TB-EPS) play a substantial role on microbial aggregates, which can promote microbial cells to aggregate and adhere onto the carrier in bioreactor. [...]

Modification of Cellulose Nanofibrils with Luminescent Carbon Dots

Publication year: 2014
Authors: Junkka K. 1, Guo J. 1, Filpponen I. 1, Lainen J. 1, Rojas O.J. 1,2
Published in: Biomacromolecules, 2014, Vol. 15 (3), p. 876–881
Films and hydrogels consisting of cellulose nanofibrils (CNF) were modified by covalent EDC/NHS coupling of luminescent, water-dispersible carbon dots (CDs). [...]

Effects of Composition of Oligo(ethylene glycol)-Based Mixed Monolayers on Peptide Grafting and Human Immunoglobulin Detection

Publication year: 2014
Authors: Islam N. †, Gurgel P.V. †, Rojas O.J. †‡, Carbonell R.G. †§
Published in: Journal of Physical Chemistry, 2014, Vol. 118 (10), p. 5361–5373
Alkanethiols carrying ethylene glycol units (EGn, n = 3 or 6) with amine termini (EG3NH2 or EG6NH2) were coadsorbed with a “diluent”, hydroxyl-terminated alkanethiol (EG3OH), to form mixed self-assembled monolayers (SAMs). The mixed SAMs were characterized, and hexameric peptide ligand His-Trp-Arg-Gly-Trp-Val (HWRGWV), which shows affinity binding toward the Fc (constant fragment) of human immunoglobulin (IgG), was grafted onto different dilutions of EG6NH2–EG3OH mixed SAMs for preparation of IgG detection surfaces. [...]

The effect of coatings on the performance of the microelectrode arrays

Publication year: 2014
Authors: Sivasubramaniapandian Manoj
Published in: Master's Thesis
Microelectrode arrays (MEAs) are pretreated with suitable surface molecules prior to culturing of cells, since the properties of the substrate have a decisive effect on the adhesion, migration, differentiation, and the growth of the cells. The purpose of this thesis is to study a few of these coating materials, and to evaluate their impact on the performance of the MEA. [...]

Triggering Protein Adsorption on Tailored Cationic Cellulose Surfaces

Publication year: 2014
Authors: Mohan T. 1, Niegelhell K. 4, Pinto Zarth C.S. 2, Kargl R. 3, Köstler S. 5, Ribitsch V. 1, Heinze T. 2, Spirk S. 1,4, Stana-Kleinschek K. 3
Published in: Biomacromolecules, 2014, Vol. 15 (11), p. 3931–3941
The equipment of cellulose ultrathin films with BSA (bovine serum albumin) via cationization of the surface by tailor-made cationic celluloses is described. In this way, matrices for controlled protein deposition are created, whereas the extent of protein affinity to these surfaces is controlled by the charge density and solubility of the tailored cationic cellulose derivative. [...]

An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes

Publication year: 2014
Authors: Ihalainen P. 1, Pettersson F. 2, Pesonen M. 2, Viitala T. 3, Määttänen A.1, Österbacka R. 2, Peltonen J. 1
Published in: Nanotechnology, 2014, Vol. 25, Issue 9
In this study, two different supramolecular recognition architectures for impedimetric detection of DNA hybridization have been formed on disposable paper-supported inkjet-printed gold electrodes. The gold electrodes were fabricated using a gold nanoparticle based ink. The first recognition architecture consists of subsequent layers of biotinylated self-assembly monolayer (SAM), streptavidin and biotinylated DNA probe. [...]

A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations

Publication year: 2014
Authors: Tang W., Hu S., Wang H., Zhao Y., Li N., Liu F.*
Published in: Chemical Communications, 2014, Vol. 50, Issue 92, p. 14352-14355
A universal molecular translator based on the target-triggered DNA strand displacement was developed, which was able to convert various kinds of non-nucleic acid targets into a unique output DNA. [...]

Self-assembly of flagellin on Au(111) surfaces

Publication year: 2014
Authors: González Orive A. 1, Pissinis D.E. 2, Diaz C. 2, Miñán A. 2, Benítez G.A. 2, Rubert A. 2, Daza Millone A. 2, Rumbo M. 3, Hernández Creus A. 1, Salvarezza R.C. 2, Schilardi P.L. 4
Published in: Journal of Colloid and Interface Science, Vol. 433 (2014), p. 86–93
The adsorption of flagellin monomers from Pseudomonas fluorescens on Au(111) has been studied by Atomic Force Microscopy (AFM), Scanning Tunneling Microscopy (STM), X-ray Photoelectron Spectroscopy (XPS), Surface Plasmon Resonance (SPR), and electrochemical techniques. [...]

Dynamic and equilibrium performance of sensors based on short peptide ligands for affinity adsorption of human IgG using surface plasmon resonance

Publication year: 2014
Authors: Islam N. 1, Shen F. 1, Gurgel P.V. 1, Rojas O.J. 2, Carbonell R.G. 3
Published in: Biosensors and Bioelectronics, Vol. 58, 2014, p. 380-387
This paper characterizes the potential of novel hexameric peptide ligands for on-line IgG detection in bioprocesses. Surface Plasmon Resonance (SPR) was used to study the binding of human IgG to the hexameric peptide ligand HWRGWV, which was covalently grafted to alkanethiol self-assembled monolayers (SAM) on gold surfaces. [...]

Control of the Morphology of Lipid Layers by Substrate Surface Chemistry

Publication year: 2014
Authors: Niko Granqvist *†, Marjo Yliperttula †, Salla Välimäki ‡, Petri Pulkkinen ‡, Heikki Tenhu ‡, and Tapani Viitala *†
Published in: Langmuir, 2014, Vol. 30 (10), p. 2799–2809
In this study, surface coatings were used to control the morphology of the deposited lipid layers during vesicle spreading, i.e., to control if liposomes self-assemble on a surface into a supported lipid bilayer or a supported vesicular layer. [...]

Effect of gold nanoparticles on the structure and electron-transfer characteristics of glucose oxidase redox polyelectrolyte-surfactant complexes

Publication year: 2014
Authors: Cortez M.L. a,b, Marmisollé W. a, Pallarola D. b, Pietrasanta L.I. c, Murgida D.H. a, Ceolín M. b, Azzaroni O. b, Battaglini F. a
Published in: Chemistry A European Journal, 2014, Vol. 20, Issue 41, p. 13037–13404
Efficient electrical communication between redox proteins and electrodes is a critical issue in the operation and development of amperometric biosensors. The present study explores the advantages of a nanostructured redox-active polyelectrolyte-surfactant complex containing [Os(bpy)2Clpy](2+) (bpy=2,2'-bipyridine, py= pyridine) as the redox centers and gold nanoparticles (AuNPs) as nanodomains for boosting the electron-transfer propagation throughout the assembled film in the presence of glucose oxidase (GOx). [...]