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Lysozyme adsorption at a silica surface using simulation and experiment: effects of pH on protein layer structure

Publication year: 2015
Authors: Karina Kubiak-Ossowska a, Monika Cwieka b, Agnieszka Kaczynska c, Barbara Jachimska b and Paul A. Mulheran, *a
Published in: Phys. Chem. Chem. Phys., 2015,17, p. 24070-24077
Hen Egg White Lysozyme (HEWL) is a widely used exemplar to study protein adsorption on surfaces and interfaces. Here we use fully atomistic Molecular Dynamics (MD) simulations, Multi-Parametric Surface Plasmon Resonance (MP-SPR), contact angle and zeta potential measurements to study [...]

Microbial attachment and adsorption–desorption kinetic of tightly bound extracellular polymeric substances on model organic surfaces

Publication year: 2015
Authors: Guo J.S. 1,2, Zhang P. 1, Chen Y.P. 1,2, Yu Shen Y. 2, Hu X. 1, Yan P. 2, Yang J-X. 2, Fang F. 1, Li C. 1, Gao X. 1, Wang G-X. 3
Published in: Chemical Engineering Journal, 2015, Vol. 279, p. 516–521
The attachment of microbial cells onto the surface is influenced by surface properties. In this study, in order to determine the bacterial attachment and adsorption behaviors of tightly bound extracellular polymeric substances (TB-EPS) on different surfaces, four self-assembled monolayers (SAMs) carrying methyl (CH3-SAM), amino (NH2-SAM), hydroxyl (OH-SAM), and carboxyl (COOH-SAM) terminal groups were modeled. [...]

Monitoring of drug release kinetics from thin polymer films by multi-parametric surface plasmon resonance

Publication year: 2015
Authors: Korhonen K, Granqvist N, Ketolainen J, Laitinen R
Published in: International Journal of Pharmaceutics, 2015, Vol. 494(1), p. 531-536
The aim of the present study is to monitor the release of perphenazine (PPZ) from thin polymer films in real-time by the multi-parametric surface plasmon resonance method (MP-SPR). The MP-SPR method is capable of measuring changes in polymer films that are significantly thicker than the apparent scanning depth of the SPR field. [...]

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Publication year: 2015
Authors: Miyazaki C.M. 1, Pereira T.P. 1, Tavares Mascagni D.B. 2, de Moraes M.L. 3, Ferreira M. 1
Published in: Materials Science and Engineering: C, 2015, Vol. 58, p. 310–315
In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. [...]

Using QCM and SPR for the Kinetic Evaluation of the Binding Between A New Recombinant Chimeric Protein and Specific Antibodies of the Visceral Leishmaniasis

Publication year: 2015
Authors: Souto D.E., Faria A.R., de Andrade H.M., Kubota L.T.
Published in: Current Protein and Peptide Science, 2015, Vol. 16, p. 782-790
In the present study, the surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) techniques were employed to kinetically evaluate the binding affinity of a new recombinant chimeric protein (CP10) toward anti-Leishmania infantum antibodies [...]

Reversible Switching of Redox-Active Molecular Orbitals and Electron Transfer Pathways in CuA Sites of Cytochrome c Oxidase

Publication year: 2015
Authors: Ulises Zitare, Damián Alvarez Paggi, Marcos N.Morgada, Luciano A.Abriata, Alejadro J.Vila, Daniel H.Murgida
Published in: Angewandte Chemie International Edition (Impact Factor: 11.26). 06/2015; 54(33)
The CuA site of cytochrome c oxidase is a redox hub that participates in rapid electron transfer at low driving forces with two redox cofactors in nearly perpendicular orientations. Spectroscopic and electrochemical characterizations [...]

SPR analysis of the interaction between a recombinant protein of unknown function in Leishmania infantum immobilised on dendrimers and antibodies of the visceral leishmaniasis: A potential use in immunodiagnosis

Publication year: 2015
Authors: Souto D.E. 1, Fonseca A.M. 2, Barragan J.T. 1, Luz Rde C. 3, Andrade H.M. 2, Damos F.S. 3, Kubota L.T. 4
Published in: Biosensors and Bioelectronics, 2015, Vol. 70, p. 275-81
In this work, an SPR immunosensor was developed to elucidate the reaction kinetics between a protein of unknown function in Leishmania infantum (hypothetical C1 protein) and specific antibodies of [...]

Strongly Stretched Protein Resistant Poly(ethylene glycol) Brushes Prepared by Grafting-To

Publication year: 2015
Authors: Gustav Emilsson †, Rafael L. Schoch ‡, Laurent Feuz , Fredrik Höök †, Roderick Y. H. Lim ‡, and Andreas B. Dahlin *†
Published in: ACS Applied Materials & Interfaces, 2015, 7 (14), pp 7505–7515
We present a new grafting-to method for resistant “non-fouling” poly(ethylene glycol) brushes, which is based on grafting of polymers with reactive end groups in 0.9 M Na2SO4 at room temperature. [...]

Structural and Viscoelastic Properties of Layer-by-Layer Extracellular Matrix (ECM) Nanofilms and Their Interactions with Living Cells

Publication year: 2015
Authors: Nishiguchi A., Matsusaki M., Akashi M.
Published in: ACS Biomaterials Science Engineering, 2015, Vol. 1[9], p. 816–824
Modulation of living cell surfaces by chemical and biological engineering and the control of cellular functions has enormous potential for immunotherapy, transplantation, and drug delivery. [...]

Surface modification of cellulose nanocrystals with cetyltrimethylammonium bromide

Publication year: 2014
Authors: Tiffany Abitbol, Heera Marway and, Emily D. Cranston Tiffany Abitbol (tiffany.abitbol@mail.mcgill.ca), Heera Marway (marwayhs@mcmaster.ca), Emily D. Cranston (ecranst@mcmaster.ca)
Published in: Special Issue: NANOCELLULOSE; Nordic Pulp & Paper Research Journal Vol 29 no (1) 2014
Cellulose nanocrystals (CNCs) prepared by sulfuric acid hydrolysis of cotton were surface modified with cetyltrimethylammonium bromide (CTAB). Essentially, the counterions of the CNC surface sulfate ester groups are exchanged for cetyltrimethyl-ammonium (CTA+), which acts as a bulky, amphiphilic cation. [...]

Photoresponsive cellulose fibers by surface modification with multifunctional cellulose derivatives

Publication year: 2014
Authors: Olga Grigoray (a), Holger Wondraczek(a), Elina Heikkilä(a), Pedro Fardim (a,b,∗), Thomas Heinze (a,c)
Published in: Carbohydrate Polymers 111 (2014) 280–287
Eucalyptus bleached kraft pulp fibers were modified by adsorption of novel bio-based multifunctionalcellulose derivatives in order to generate light responsive surfaces. [...]

Modification of surfaces with adsorption of amphiphilic polymers

Publication year: 2014
Authors: Katri Kontturi
Published in: Doctoral Dissertation
In this work the adsorption of amphiphilic polymers and their utilization in the modification of planar surfaces were examined. The approach was to systematically explore adsorption of very different types of [...]

Surface Plasmon Resonance in Living Cell Sensing

Publication year: 2014
Authors: Teemu Suutari
Published in: Master's Thesis
Surface plasmon resonance (SPR) is a label free technique to study surface interactions. It is based on photon-plasmon coupling. Laser light is directed through a prism and reflects form a metal surface, often gold. At certain conditions, photons turn into plasmons, which then propagate [...]

Marine Biofouling Resistance of Polyurethane with Biodegradation and Hydrolyzation

Publication year: 2014
Authors: Wentao Xu,† Chunfeng Ma,*,† Jielin Ma,† Tiansheng Gan,† and Guangzhao Zhang*,†,‡
Published in: ACS Applied Materials & Interfaces, 2014
We have prepared polyurethane with poly(ε-caprolactone) (PCL) as the segments of the main chain and poly(triisopropylsilyl acrylate) (PTIPSA) as the side chains by a combination of radical polymerization and a condensation reaction. [...]

Investigation of pH-Induced Protein Conformation Changes by Nanomechanical Deflection

Publication year: 2014
Authors: Thakur G. †, Jiang K. †, Lee D. †, Prashanthi K. †, Kim S. †‡, Thundat T. †
Published in: Langmuir, 2014, Vol. 30 (8), p. 2109–2116
Broad-spectrum biosensing technologies examine sensor signals using biomarkers, such as proteins, DNA, antibodies, specific cells, and macromolecules, based on direct- or indirect-conformational changes. [...]

Grafting hyaluronic acid onto gold surface to achieve low protein fouling in surface plasmon resonance biosensors

Publication year: 2014
Authors: Liu X., Huang R., Su R., Qi W., Wang L., He Z.
Published in: ACS Applied Materials and Interfaces, 2014, Vol. 6(15), p. 13034-42
Antifouling surfaces capable of reducing nonspecific protein adsorption from natural complex media are highly desirable in surface plasmon resonance (SPR) biosensors. A new protein-resistant surface [...]

Adsorption behavior of tightly bound extracellular polymeric substances on model organic surfaces under different pH and cations with surface plasmon resonance

Publication year: 2014
Authors: Zhang P. 1, Chen Y.P. 2, Guo J.S. 3, Shen Y. 4, Yang J.X. 4, Fang F. 1, Li C. 1, Gao X. 1, Wang G.X. 5
Published in: Water Research, 2014, Vol.57, p. 31-9
Tightly bound extracellular polymeric substances (TB-EPS) play a substantial role on microbial aggregates, which can promote microbial cells to aggregate and adhere onto the carrier in bioreactor. [...]

Modification of Cellulose Nanofibrils with Luminescent Carbon Dots

Publication year: 2014
Authors: Junkka K. 1, Guo J. 1, Filpponen I. 1, Lainen J. 1, Rojas O.J. 1,2
Published in: Biomacromolecules, 2014, Vol. 15 (3), p. 876–881
Films and hydrogels consisting of cellulose nanofibrils (CNF) were modified by covalent EDC/NHS coupling of luminescent, water-dispersible carbon dots (CDs). [...]

Effects of Composition of Oligo(ethylene glycol)-Based Mixed Monolayers on Peptide Grafting and Human Immunoglobulin Detection

Publication year: 2014
Authors: Islam N. †, Gurgel P.V. †, Rojas O.J. †‡, Carbonell R.G. †§
Published in: Journal of Physical Chemistry, 2014, Vol. 118 (10), p. 5361–5373
Alkanethiols carrying ethylene glycol units (EGn, n = 3 or 6) with amine termini (EG3NH2 or EG6NH2) were coadsorbed with a “diluent”, hydroxyl-terminated alkanethiol (EG3OH), to form mixed self-assembled monolayers (SAMs). The mixed SAMs were characterized, and hexameric peptide ligand His-Trp-Arg-Gly-Trp-Val (HWRGWV), which shows affinity binding toward the Fc (constant fragment) of human immunoglobulin (IgG), was grafted onto different dilutions of EG6NH2–EG3OH mixed SAMs for preparation of IgG detection surfaces. [...]

The effect of coatings on the performance of the microelectrode arrays

Publication year: 2014
Authors: Sivasubramaniapandian Manoj
Published in: Master's Thesis
Microelectrode arrays (MEAs) are pretreated with suitable surface molecules prior to culturing of cells, since the properties of the substrate have a decisive effect on the adhesion, migration, differentiation, and the growth of the cells. The purpose of this thesis is to study a few of these coating materials, and to evaluate their impact on the performance of the MEA. [...]