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Interactions between Cellulolytic Enzymes with Native, Autohydrolysis, and Technical Lignins and the Effect of a Polysorbate Amphiphile in Reducing Nonproductive Binding

Publication year: 2015
Authors: Fritz C. 1, Ferrer A. 1, Salas C. 1, Jameel H. 1, Rojas O.J. 1,2
Published in: Biomacromolecules, 2015, Vol. 16(12), p. 3878-88
Understanding enzyme-substrate interactions is critical in designing strategies for bioconversion of lignocellulosic biomass. In this study we monitored molecular events, in situ and in real time, including the adsorption and desorption of cellulolytic enzymes on lignins and cellulose, by using quartz crystal microgravimetry and surface plasmon resonance. [...]

Cholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes

Publication year: 2015
Authors: Alm I., García-Linares S., Gavilanes J.G., Martínez-Del-Pozo Á., Slotte J.P.
Published in: Biochimica et Biophysica Acta, 2015, Vol. 1848(4), p. 925-31
The pore forming capacity of Sticholysin II (StnII; isolated from Stichodactyla helianthus) in bilayer membranes containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), palmitoylsphingomyelin (PSM) and either cholesterol or palmitoyl ceramide (PCer) has been examined. The aim of the study was to elucidate how the presence of differently ordered PSM domains affected StnII oligomerization and pore formation. [...]

UV crosslinked poly(acrylic acid): a simple method to bio-functionalize electrolyte-gated OFET biosensors

Publication year: 2015
Authors: M. Y. Mulla a, P. Seshadri a, L. Torsi a, K. Manoli a, A. Mallardi b, N. Ditaranto a, M. V. Santacroce c, C. Di Franco c, G. Scamarcio c and M. Magliulo, *a
Published in: Journal of Materials Chemistry B, 2015, Vol. 3, p. 5049-5057
A simple and time-saving wet method to endow the surface of organic semiconductor films with carboxyl functional groups is presented. A thin layer of poly(acrylic acid) (pAA) is spin-coated directly on the electronic channel of [...]

Oncolytic adenoviruses coated with MHC-I tumor epitopes increase the anti-tumor immunity and efficacy against melanoma

Publication year: 2015
Authors: Cristian Capasso a, Mari Hirvinen a, Mariangela Garofalo a,c, Dmitrii Romaniuk a, Lukasz Kuryk a, Teea Sarvela a, Andrea Vitale d, Maxim Antopolsky b, Aniket Magarkar b, Tapani Viitala b, Teemu Suutari b, Alex Bunker b, Marjo Yliperttula b, Arto Urtti b,e & Vincenzo Cerullo a*
Published in: Taylor & Francis Online, 2015, article n. e1105429
The stimulation of the immune system using oncolytic adenoviruses (OAds) has attracted significant interest and several studies suggested that OAd´s immunogenicity might be important for their efficacy. Therefore, we developed a versatile and rapid system [...]

pH-responsive ion transport in polyelectrolyte multilayers of poly(diallyldimethylammonium chloride) (PDADMAC) and poly(4-styrenesulfonic acid-co-maleic acid) (PSS-MA) bearing strong- and weak anionic groups

Publication year: 2015
Authors: Maza E. a, Tuninetti J.S. a, Politakos N. b, Knoll W. c, Moya S. b, Azzaroni O. *a
Published in: Physical Chemistry Chemical Physics, 2015,Vol. 17, p. 29935-29948
The layer-by-layer construction of interfacial architectures displaying stimuli-responsive control of mass transport is attracting increasing interest in materials science. In this work, we describe the creation of interfacial architectures [...]

Degradable Polymer with Protein Resistance in a Marine Environment

Publication year: 2015
Authors: Ma J1, Ma C*1, and Zhang G*1,2
Published in: Langmuir, 2015, Vol. 31 [23], p. 6471–6478
Protein resistance is the central issue in marine antibiofouling. We have prepared poly(ε-caprolactone) (PCL)-based polyurethane with 2-(dimethylamino) ethyl methacrylate (DEM) as pendant groups [...]

Real-time Detection of Breast Cancer Cells Using Peptide-functionalized Microcantilever Arrays

Publication year: 2015
Authors: Hashem Etayash 1,2, Keren Jiang 2, Sarfuddin Azmi 1, Thomas Thundat 2, Kamaljit Kaura 1
Published in: Scientific Reports, 2015, Vol. 5, p. 13967
Ligand-directed targeting and capturing of cancer cells is a new approach for detecting circulating tumor cells (CTCs). Ligands such as antibodies have been successfully used for capturing cancer cells and an antibody based system (CellSearch®) is currently used [...]

Surface Dynamics Study of DNA Loop Formation Using Surface Plasmon Resonance

Publication year: 2015
Authors: Mary Arugula, Fuling Yang, Subramaniam Somasundaram, Christopher J Easley, Curtis Shannon and Aleksandr Simonian
Published in: ECS Transactions, 2015, Vol. 66, Issue 36, p. 19-29
Electrochemical proximity assay (ECPA), was recently developed for direct protein quantitation as low as 158 fM. In this assay, the loop-like DNA structure holds methylene blue (MB-DNA) in close proximity with the gold surface when target is present, ensuring efficient electron transfer. However, for studying DNA binding [...]

Effects of charge ratios of xylan-poly(allylamine hydrochloride) complexes on their adsorption onto different surfaces

Publication year: 2015
Authors: Galván MV1,Soledad Peresin M2, Mocchiutti P1, Granqvist N3, Zanuttini MA1, Tammelin T2
Published in: Cellulose, 2015, 22 [5], p. 2955-2970
The adsorption behavior of polyelectrolyte complexes (PECs) of poly(allylamine hydrochloride) (PAH) and 4-O-methylglucuronoxylan (Xyl) onto silica surface, cellulose nanofibrils (CNFs) model surfaces, and unbleached softwood kraft fibers was studied. Different charge ratios and ionic strengths of the liquid medium (q − /q +: 0.3, 0.5 and 0.8 in 1 mM NaCl, and q − /q +: 0.2, 0.3 and 0.5 in 10 mM NaCl), at pH 7.5 were considered. [...]

Biosensors for Detecting Genetically Modified Organisms in Food and Feed

Publication year: 2015
Authors: Mary Arugula, Alina Chanysheva, Kiril Vaglenov and Aleksandr Simonian
Published in: ECS Transactions, 2015, Vol. 66, Issue 36, p. 31-38
The detection of genetically modified organisms is of growing interest given their considerable presence in vast variety of food and feed ingredients, as well as their environmental impact. In this study we demonstrate real-time, label- free detection of DNA from genetically modified, Roundup Ready (RR) soybeans by surface plasmon resonance (SPR). [...]

Effect of Molecular Architecture of PDMAEMA-POEGMA Random and Block Copolymers on Their Adsorption on Regenerated and Anionic Nanocelluloses and Evidence of Interfacial Water Expulsion

Publication year: 2015
Authors: Vuoriluoto M. 1, Orelma H. 1,2, Johansson L.S. 1, Zhu B. 3, Poutanen M. 4, Walther A. 3, Laine J. 1, Rojas O.J. 1
Published in: Journal of Physical Chemistry, 2015, Vol. 119, p. 15275-86
Block copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) with varying block sizes were synthesized by consecutive reversible addition-fragmentation chain transfer (RAFT) polymerization and then exposed to cellulose substrates with different anionic charge density. [...]

Functional nicotinic acetylcholine receptor reconstitution in Au(111)-supported thiolipid monolayers

Publication year: 2015
Authors: Pissinis D.E. 1, Diaz C., Maza E., Bonini I.C., Barrantes F.J., Salvarezza R.C., Schilardi P.L.
Published in: Nanoscale, 2015, Vol. 7(38), p. 15789-97
The insertion and function of the muscle-type nicotinic acetylcholine receptor (nAChR) in Au(111)-supported thiolipid self-assembled monolayers have been studied by atomic force microscopy (AFM), surface plasmon resonance (SPR), and [...]

Interaction studies between indomethacin nanocrystals and PEO/PPO copolymer stabilizers

Publication year: 2015
Authors: Liu P, Viitala T., Kartal-Hodzic A., Liang H., Laaksonen T., Hirvonen J., Peltonen L.
Published in: Pharmaceutical research, 2015, Vol. 32(2), p. 628-39
PURPOSE: The lack of effective screening methods and systemic understanding of interaction mechanisms complicates the stabilizer selection process for nanocrystallization. This study focuses on the efficiency of stabilizers with various molecular compositions and structures to stabilize drug nanocrystals. [...]

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces

Publication year: 2015
Authors: Zhou L. a, Arugula M.A. a, Easley C. b, Shannon C. b, Simonian A.L. a
Published in: ECS Transactions, 2015, Vol. 69 (38), p. 9-16
With increasing significance of characterization in functional interfaces, a Multi-Parametric/Multimodal Spectroscopy Apparatus was proposed here to enhance the power of interface characterization sensing techniques. [...]

Investigation of cell behaviors on thermo-responsive PNIPAM microgel films

Publication year: 2015
Authors: Wei J. 1, Cai J. 1, Li Y. 1, Wu B. 2, Gong X. 2, Ngai T. 1
Published in: Colloids and Surfaces B: Biointerfaces, 2015, Vol. 132, p. 202–207
The use of poly(N-isopropylacrylamide) (PNIPAM) as building blocks for engineering responsive coatings and their potential use as switchable substrates such as biosensors have attracted great attention in recent years. [...]

Lysozyme adsorption at a silica surface using simulation and experiment: effects of pH on protein layer structure

Publication year: 2015
Authors: Karina Kubiak-Ossowska a, Monika Cwieka b, Agnieszka Kaczynska c, Barbara Jachimska b and Paul A. Mulheran, *a
Published in: Phys. Chem. Chem. Phys., 2015,17, p. 24070-24077
Hen Egg White Lysozyme (HEWL) is a widely used exemplar to study protein adsorption on surfaces and interfaces. Here we use fully atomistic Molecular Dynamics (MD) simulations, Multi-Parametric Surface Plasmon Resonance (MP-SPR), contact angle and zeta potential measurements to study [...]

Microbial attachment and adsorption–desorption kinetic of tightly bound extracellular polymeric substances on model organic surfaces

Publication year: 2015
Authors: Guo J.S. 1,2, Zhang P. 1, Chen Y.P. 1,2, Yu Shen Y. 2, Hu X. 1, Yan P. 2, Yang J-X. 2, Fang F. 1, Li C. 1, Gao X. 1, Wang G-X. 3
Published in: Chemical Engineering Journal, 2015, Vol. 279, p. 516–521
The attachment of microbial cells onto the surface is influenced by surface properties. In this study, in order to determine the bacterial attachment and adsorption behaviors of tightly bound extracellular polymeric substances (TB-EPS) on different surfaces, four self-assembled monolayers (SAMs) carrying methyl (CH3-SAM), amino (NH2-SAM), hydroxyl (OH-SAM), and carboxyl (COOH-SAM) terminal groups were modeled. [...]

Monitoring of drug release kinetics from thin polymer films by multi-parametric surface plasmon resonance

Publication year: 2015
Authors: Korhonen K, Granqvist N, Ketolainen J, Laitinen R
Published in: International Journal of Pharmaceutics, 2015, Vol. 494(1), p. 531-536
The aim of the present study is to monitor the release of perphenazine (PPZ) from thin polymer films in real-time by the multi-parametric surface plasmon resonance method (MP-SPR). The MP-SPR method is capable of measuring changes in polymer films that are significantly thicker than the apparent scanning depth of the SPR field. [...]

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Publication year: 2015
Authors: Miyazaki C.M. 1, Pereira T.P. 1, Tavares Mascagni D.B. 2, de Moraes M.L. 3, Ferreira M. 1
Published in: Materials Science and Engineering: C, 2015, Vol. 58, p. 310–315
In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. [...]

Using QCM and SPR for the Kinetic Evaluation of the Binding Between A New Recombinant Chimeric Protein and Specific Antibodies of the Visceral Leishmaniasis

Publication year: 2015
Authors: Souto D.E., Faria A.R., de Andrade H.M., Kubota L.T.
Published in: Current Protein and Peptide Science, 2015, Vol. 16, p. 782-790
In the present study, the surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) techniques were employed to kinetically evaluate the binding affinity of a new recombinant chimeric protein (CP10) toward anti-Leishmania infantum antibodies [...]