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Assembly of citrate gold nanoparticles on hydrophilic monolayers

Publication year: 2016
Authors: Vikholm-Lundin I. a, b, Rosqvist E. c, Ihalainen P. c, Munter T. d, Honkimaa A. e, Marjomäki V. f, Albers W.M. g, Peltonen J. c
Published in: Applied Surface Science, 2016, Vol. 378, p. 519–529
Self-assembled monolayers (SAMs) as model surfaces were linked onto planar gold films thorough lipoic acid or disulfide groups. The molecules used were polyethylene glycol (EG-S-S), N-[tris-(hydroxymethyl)methyl]acrylamide polymers with and without lipoic acid (Lipa-pTHMMAA and pTHMMAA) and a lipoic acid triazine derivative (Lipa-MF). [...]

Controlled Immobilization Strategies to Probe Short Hyaluronan-Protein Interactions

Publication year: 2016
Authors: Burcu Baykal Minsky 1,2, Christiane H. Antoni 1,2, Heike Boehm 1,2,3
Published in: Nature Scientific Reports, 2016, Vol. 6, article n. 21608
Well-controlled grafting of small hyaluronan oligosaccharides (sHA) enables novel approaches to investigate biological processes such as angiogenesis, immune reactions and cancer metastasis. [...]

Noncovalent functionalization of solid-state nanopores via self-assembly of amphipols

Publication year: 2016
Authors: Pérez-Mitta G. 1, Burr L. 2, Tuninetti J.S. 1, Trautmann C. 2, Toimil-Molares M.E. 3, Azzaroni O. 4
Published in: Nanoscale, 2016, Vol. 8(3), p. 1470-8
In recent years there has been increasing interest in the development of new methods for conferring functional features to nanopore-based fluidic devices. In this work, we describe for the first time the noncovalent integration of amphoteric-amphipathic polymers, also known as "amphipols", into single conical nanopores in order to obtain signal-responsive chemical nanodevices. [...]

Toxin-induced pore formation is hindered by intermolecular hydrogen bonding in sphingomyelin bilayers

Publication year: 2016
Authors: Sara García-Linares a, b, Juan Palacios-Ortega a,b, Tomokazu Yasuda b,c, Mia Åstrand b, José G. Gavilanes a, Álvaro Martínez-del-Pozo a, J. Peter Slotte b
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1858, Issue 6, p. 1189–1195
Sticholysin I and II (StnI and StnII) are pore-forming toxins that use sphingomyelin (SM) for membrane binding. We examined how hydrogen bonding among membrane SMs affected the StnI- and StnII-induced pore formation process, resulting in bilayer permeabilization. [...]

Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers

Publication year: 2016
Authors: Vuoriluoto M. 1, Orelma H. 1, Zhu B. 2, Johansson L.S. 1, Rojas O.J. 1
Published in: ACS Applied Materials & Interfaces, 2016, 2;8 (8), p: 5668-78
We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). [...]

Regulation of Sticholysin II-Induced Pore Formation by Lipid Bilayer Composition, Phase State, and Interfacial Properties

Publication year: 2016
Authors: Palacios-Ortega J. 1,2, García-Linares S. 1,2, Åstrand M. 2, Al Sazzad M.A. 2, Gavilanes J.G. 1, Martínez-Del-Pozo Á. 1, Slotte J.P. 2
Published in: Langmuir, 2016, 32(14), p. 3476-84
Sticholysin II (StnII) is a pore-forming toxin that uses sphingomyelin (SM) as the recognition molecule in targeting membranes. After StnII monomers bind to SM, several toxin monomers act in concert to oligomerize into a functional pore. [...]

Cationic and anionic polyelectrolyte complexes of xylan and chitosan. Interaction with lignocellulosic surfaces

Publication year: 2016
Authors: Mocchiutti P. 1, Schnell C.N. 2, Rossi G.D. 3, Peresin M.S. 4, Zanuttini M.A. 5, Galván M.V. 6
Published in: Carbohydrate Polymers, Vol. 150, p.89-98
Cationic (CatPECs) and anionic (AnPECs) polyelectrolyte complexes from xylan and chitosan were formed, characterized and adsorbed onto unbleached fibers for improving the papermaking properties.They were prepared [...]

On-line coupling of surface plasmon resonance optical sensing to size-exclusion chromatography for affinity assessment of antibody samples

Publication year: 2016
Authors: Dina Lakayan a, b, Rob Haselberg a, Wilfried M.A. Niessen a, c, Govert W. Somsen a, Jeroen Kool a
Published in: Journal of Chromatography A, Vol. 1452, p. 81–88
Surface plasmon resonance (SPR) is an optical technique that measures biomolecular interactions. Stand-alone SPR cannot distinguish different binding components present in one sample. Moreover, sample matrix components may show non-specific binding to the sensor surface [...]

Combining Surface Plasmon Resonance and Quartz Crystal Microbalance To Determine Hydration of Dendrimer Monolayers

Publication year: 2016
Authors: Barbara Jachimska and Karolina Tokarczyk
Published in: Journal of Physical Chemistry C
Polyelectrolytes are abundant in nature and crucial due to their versatility in biological systems. The controlled assembly of polyelectrolytes has potential applications in the formation of nanostructured and microstructured materials of desired structure and functionality. [...]

Immunological boosting and personalization of oncolytic virotherapies for cancer treatment

Publication year: 2016
Authors: Mari Hirvinen
Published in: Doctoral Dissertation
Cancer is the leading cause of death worldwide creating a need for novel cancer treatments that are more efficient but also safer and more specific. Oncolytic viruses (OVs) have shown a solid safety profile in clinical trials. OVs are nowadays considered immunotherapies because of to their ability to stimulate the host immune system to fight against cancer. [...]

Adsorption Studies of Organophosphonic Acids on Differently Activated Gold Surfaces

Publication year: 2016
Authors: Katrin Niegelhell †, Simon Leimgruber †‡, Thomas Grießer §, Christian Brandl ∥, Boril Chernev ∥, Robert Schennach ⊥, Gregor Trimmel †, and Stefan Spirk †
Published in: Langmuir, 2016, Vol. 32 (6), p. 1550–1559
In this study, the formation of self-assembled monolayers consisting of three organophosphonic acids (vinyl-, octyl-, and tetradecylphosphonic acid) from isopropanol solutions onto differently activated gold surfaces is studied in situ and in real time using multiparameter surface plasmon resonance (MP-SPR). [...]

Real-Time Protein and Cell Binding Measurements on Hydroxyapatite Coatings

Publication year: 2016
Authors: A. M. Vilardell 1 , N. Cinca 1 , A. Jokinen 2, N. Garcia-Giralt 3, S. Dosta 1, I. G. Cano 1 and J. M. Guilemany 1
Published in: Journal of Functional Biomaterials, 2016, Vol. 7(3), p. 23
Although a lot of in vitro and in vivo assays have been performed during the last few decades years for hydroxyapatite bioactive coatings, there is a lack of exploitation of real-time in vitro interaction measurements. In the present work, real-time interactions for a plasma sprayed hydroxyapatite coating were measured by a Multi-Parametric Surface Plasmon Resonance (MP-SPR), [...]

Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation

Publication year: 2016
Authors: Otto K. Kari 1, Tatu Rojalin 2, Stefano Salmaso 3, Michela Barattin 4, Hanna Jarva 5, Seppo Meri 6, Marjo Yliperttula 7, Tapani Viitala 8, Arto Urtti 9
Published in: Drug Delivery and Translational Research, p. 1-13
When nanocarriers are administered into the blood circulation, a complex biomolecular layer known as the “protein corona” associates with their surface. Although the drivers of corona formation are not known, it is widely accepted that this layer mediates biological interactions of the nanocarrier with its surroundings. [...]

Topography effects in AFM force mapping experiments on xylan-decorated cellulose thin films

Publication year: 2016
Authors: Ganser Ch. 1,2,3, Niegelhell K. 4, Czibula C. 1,3, Chemelli A. 5, Teichert Ch. 1,3, Schennach R. 3,6, Spirk S. 4,7
Published in: International Journal of the Biology, Chemistry, Physics and Technology of Woo
Xylan-coated cellulose thin films has been investigated by means of atomic force microscopy (AFM) and force mapping experiments. The birch xylan deposition on the film was performed under control [...]

Adsorption of Xyloglucan onto Cellulose Surfaces of Different Morphologies: An Entropy-Driven Process

Publication year: 2016
Authors: Tobias Benselfelt 1, Emily D. Cranston 2, Sedat Ondaral 3, Erik Johansson 4, Harry Brumer 5, Mark W. Rutland 6, and Lars Wågberg 1
Published in: Biomacromolecules, 2016, Vol. 17(9), p. 2801-11
The temperature-dependence of xyloglucan (XG) adsorption onto smooth cellulose model films regenerated from N-methylmorpholine N-oxide (NMMO) was investigated using surface plasmon resonance spectroscopy, and it was found that the adsorbed amount increased with increasing temperature. [...]

Reversible Switching of Redox-Active Molecular Orbitals and Electron Transfer Pathways in CuA Sites of Cytochrome c Oxidase

Publication year: 2015
Authors: Ulises Zitare, Damián Alvarez Paggi, Marcos N.Morgada, Luciano A.Abriata, Alejadro J.Vila, Daniel H.Murgida
Published in: Angewandte Chemie International Edition (Impact Factor: 11.26). 06/2015; 54(33)
The CuA site of cytochrome c oxidase is a redox hub that participates in rapid electron transfer at low driving forces with two redox cofactors in nearly perpendicular orientations. Spectroscopic and electrochemical characterizations [...]

Lysozyme adsorption at a silica surface using simulation and experiment: effects of pH on protein layer structure

Publication year: 2015
Authors: Karina Kubiak-Ossowska a, Monika Cwieka b, Agnieszka Kaczynska c, Barbara Jachimska b and Paul A. Mulheran, *a
Published in: Phys. Chem. Chem. Phys., 2015,17, p. 24070-24077
Hen Egg White Lysozyme (HEWL) is a widely used exemplar to study protein adsorption on surfaces and interfaces. Here we use fully atomistic Molecular Dynamics (MD) simulations, Multi-Parametric Surface Plasmon Resonance (MP-SPR), contact angle and zeta potential measurements to study [...]

Chelating Surfaces for Native State Proteins Patterning: The Human Serum Albumin Case

Publication year: 2015
Authors: Giamblanco N., Tuccitto N., Zappalà G., Sfuncia G., Licciardello A., Marletta G.
Published in: ACS Applied Materials & Interfaces, 2015, Vol. 7 (41), p. 23353–23363
The paper reports a new “soft” surface functionalization strategy, based on a highly selective ion metal chelation process. The proposed stepwise methodology implies at first the construction of a monolayer of terpyridine-based thiol (Tpy), whose highly packed [...]

Effects of charge ratios of xylan-poly(allylamine hydrochloride) complexes on their adsorption onto different surfaces

Publication year: 2015
Authors: Galván MV1,Soledad Peresin M2, Mocchiutti P1, Granqvist N3, Zanuttini MA1, Tammelin T2
Published in: Cellulose, 2015, 22 [5], p. 2955-2970
The adsorption behavior of polyelectrolyte complexes (PECs) of poly(allylamine hydrochloride) (PAH) and 4-O-methylglucuronoxylan (Xyl) onto silica surface, cellulose nanofibrils (CNFs) model surfaces, and unbleached softwood kraft fibers was studied. Different charge ratios and ionic strengths of the liquid medium (q − /q +: 0.3, 0.5 and 0.8 in 1 mM NaCl, and q − /q +: 0.2, 0.3 and 0.5 in 10 mM NaCl), at pH 7.5 were considered. [...]

pH-responsive ion transport in polyelectrolyte multilayers of poly(diallyldimethylammonium chloride) (PDADMAC) and poly(4-styrenesulfonic acid-co-maleic acid) (PSS-MA) bearing strong- and weak anionic groups

Publication year: 2015
Authors: Maza E. a, Tuninetti J.S. a, Politakos N. b, Knoll W. c, Moya S. b, Azzaroni O. *a
Published in: Physical Chemistry Chemical Physics, 2015,Vol. 17, p. 29935-29948
The layer-by-layer construction of interfacial architectures displaying stimuli-responsive control of mass transport is attracting increasing interest in materials science. In this work, we describe the creation of interfacial architectures [...]