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Determining Number Concentrations and Diameters of Polystyrene Particles by Measuring the Effective Refractive Index of Colloids Using Surface Plasmon Resonance

Publication year: 2016
Authors: Tuoriniemi J. 1, Moreira B. 1, Safina G. 1,2
Published in: Langmuir, 2016, Vol. 32 (41), p. 10632–10640
The capabilities of surface plasmon resonance (SPR) for characterization of colloidal particles were evaluated for 100, 300, and 460 nm nominal diameter polystyrene (PS) latexes. First the accuracy of measuring [...]

Enzymes as Biodevelopers for Nano- And Micropatterned Bicomponent Biopolymer Thin Films

Publication year: 2016
Authors: Niegelhell K. 1, Süßenbacher M. 1, Jammernegg K. 1, Ganner T. 2, Schwendenwein D. 3, Schwab H. 3, Stelzer F. 1, Plank H. 2, Spirk S. 1,4
Published in: Biomacromolecules, 2016, Vol. 17 (11), p. 3743–3749
The creation of nano- and micropatterned polymer films is a crucial step for innumerous applications in science and technology. However, there are several problems associated with environmental aspects concerning the polymer synthesis itself, cross-linkers to induce the patterns as well as toxic solvents [...]

Role of the Tryptophan Residues in the Specific Interaction of the Sea Anemone Stichodactyla helianthus’s Actinoporin Sticholysin II with Biological Membranes

Publication year: 2016
Authors: García-Linares S. 1, 2, Maula T. 2, Rivera-de-Torre E. 1, Gavilanes J.G. 1, Slotte J.P. 2, Martínez-del-Pozo A. 1
Published in: Biochemistry, 2016, Vol. 55 (46), p. 6406–6420
Actinoporins are pore-forming toxins from sea anemones. Upon interaction with sphingomyelin-containing bilayers, they become integral oligomeric membrane structures that form a pore. [...]

Using electrochemical surface plasmon resonance for in-situ kinetic investigations of solid electrolyte interphase formation in lithium ion battery

Publication year: 2016
Authors: Pradanawati S.A. a, Wang F.-M. a, b, Su Ch.-H. c
Published in: Journal of Power Sources, Vol. 330, 2016, p. 127–131
The solid electrolyte interphase (SEI) significantly affects the energy density and safety performance of lithium ion batteries. Previous studies have shown that conventional analyses cannot characterize the real-time [...]

Lignocellulosic Materials and their Interactions with Proteins and Surface Active Molecules

Publication year: 2016
Authors: Fritz C.
Published in: Doctoral dissertation, North Carolina State University
Lignocellulosic biomass is a highly valuable resource for the development of value-added biobased chemicals and other materials. However, the efficient utilization of this biomass regards on understanding the different interactions [...]

Phenylboronic-Acid-Based Polymeric Micelles for Mucoadhesive Anterior Segment Ocular Drug Delivery

Publication year: 2016
Authors: Prosperi-Porta G. 1, Kedzior S. 2, Muirhead B. 1, Sheardown H. 1,2
Published in: Biomacromolecules, 2016, Vol. 17(4), p. 1449-57
Topical drug delivery to the front of the eye is extremely inefficient due to effective natural protection mechanisms such as precorneal tear turnover and the relative impermeability of the cornea and sclera tissues. This causes low ocular drug bioavailability, requiring large frequent doses that result in high systemic exposure and side effects.[...]

Dual-Wavelength Surface Plasmon Resonance for Determining the Size and Concentration of Sub-Populations of Extracellular Vesicles

Publication year: 2016
Authors: Rupert D.L.M. 1, Shelke G.V. 2, Emilsson G. 1, Claudio V. 1, Block S. 1, Lässer C. 2, Dahlin A. 1, Lötvall J.O. 2, Bally M. 1,3, Zhdanov V.P. 1,4, Höök F. 1
Published in: Analytical Chemistry, 2016, Vol. 88 (20), p. 9980–9988
Accurate concentration determination of subpopulations of extracellular vesicles (EVs), such as exosomes, is of importance both in the context of understanding their fundamental biological role [...]

Surface plasmon resonance for characterization of large-area atomic-layer graphene film

Publication year: 2016
Authors: H. Jussila 1,*, H. Yang 1, N. Granqvist 2, Z. Sun 1
Published in: Optica, Vol. 3, Issue 2, p. 151-158
Characterization of large-area thin films with atomic-scale resolution is challenging but in great demand for diverse applications (e.g., nanotechnology and sensing). Here, we use the Surface Plasmon Resonance (SPR) method to characterize both the thickness and refractive index of chemical-vapor-deposition (CVD)-grown graphene films. [...]

Raman Spectrocopy and surface plasmon resonance as photonic tools for biopharmaceutical applications

Publication year: 2016
Authors: Rojalin Tatu
Published in: Doctoral dissertation (article-based), University of Helsinki
Biophotonics is an emerging area of scientific research that uses light of photons to probe biological specimens, such as tissues, cells and molecules. The field of biophotonics is broad and considerably multidisciplinary. [...]

Controlled Immobilization Strategies to Probe Short Hyaluronan-Protein Interactions

Publication year: 2016
Authors: Burcu Baykal Minsky 1,2, Christiane H. Antoni 1,2, Heike Boehm 1,2,3
Published in: Nature Scientific Reports, 2016, Vol. 6, article n. 21608
Well-controlled grafting of small hyaluronan oligosaccharides (sHA) enables novel approaches to investigate biological processes such as angiogenesis, immune reactions and cancer metastasis. [...]

Adsorption Studies of Organophosphonic Acids on Differently Activated Gold Surfaces

Publication year: 2016
Authors: Katrin Niegelhell †, Simon Leimgruber †‡, Thomas Grießer §, Christian Brandl ∥, Boril Chernev ∥, Robert Schennach ⊥, Gregor Trimmel †, and Stefan Spirk †
Published in: Langmuir, 2016, Vol. 32 (6), p. 1550–1559
In this study, the formation of self-assembled monolayers consisting of three organophosphonic acids (vinyl-, octyl-, and tetradecylphosphonic acid) from isopropanol solutions onto differently activated gold surfaces is studied in situ and in real time using multiparameter surface plasmon resonance (MP-SPR). [...]

Influence of the sulfation degree of glycosaminoglycans on their multilayer assembly with poly-l-lysine

Publication year: 2016
Authors: Teixeira R. 1,2, Reis R.L. 1,2, Pashkuleva I. 1,2
Published in: Colloids and Surfaces B: Biointerfaces, 2016, Vol. 145, p. 567-575
We report on the build-up and the intrinsic properties of polyelectrolyte multilayer films from poly-l-lysine and glycosaminoglycans (GAGs) with different sulfation degree, i.e. different charge [...]

Toxin-induced pore formation is hindered by intermolecular hydrogen bonding in sphingomyelin bilayers

Publication year: 2016
Authors: Sara García-Linares a, b, Juan Palacios-Ortega a,b, Tomokazu Yasuda b,c, Mia Åstrand b, José G. Gavilanes a, Álvaro Martínez-del-Pozo a, J. Peter Slotte b
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1858, Issue 6, p. 1189–1195
Sticholysin I and II (StnI and StnII) are pore-forming toxins that use sphingomyelin (SM) for membrane binding. We examined how hydrogen bonding among membrane SMs affected the StnI- and StnII-induced pore formation process, resulting in bilayer permeabilization. [...]

Dopamine-assisted deposition and zwitteration of hyaluronic acid for the nanoscale fabrication of low-fouling surfaces

Publication year: 2016
Authors: Huijun Ye b, Yinqiang Xia b, Zhiqiang Liu b, Renliang Huang *a, Rongxin Su *b,c,d, Wei Qi b,c,d, Libing Wang *e, Zhimin He b
Published in: Journal of Materials Chemistry B, 2016, Vol. 4, p. 4084-4091
In this study, we proposed a bioinspired approach for the deposition and zwitteration of hyaluronic acid (HA) with a reduced glutathione (GSH) to form a composite layer that functions as a low fouling coating. [...]

Regulation of Sticholysin II-Induced Pore Formation by Lipid Bilayer Composition, Phase State, and Interfacial Properties

Publication year: 2016
Authors: Palacios-Ortega J. 1,2, García-Linares S. 1,2, Åstrand M. 2, Al Sazzad M.A. 2, Gavilanes J.G. 1, Martínez-Del-Pozo Á. 1, Slotte J.P. 2
Published in: Langmuir, 2016, 32(14), p. 3476-84
Sticholysin II (StnII) is a pore-forming toxin that uses sphingomyelin (SM) as the recognition molecule in targeting membranes. After StnII monomers bind to SM, several toxin monomers act in concert to oligomerize into a functional pore. [...]

Label-free C-reactive protein electronic detection with an electrolyte-gated organic field-effect transistor-based immunosensor

Publication year: 2016
Authors: Magliulo M. 1, De Tullio D. 2, Vikholm-Lundin I. 3, Albers W.M. 4, Munter T. 5, Manoli K. 2, Palazzo G. 2, Torsi L. 2
Published in: Analytical and Bioanalytical Chemistry, 2016, Vol. 408(15), p. 3943-52
In this contribution, we propose a label-free immunosensor, based on a novel type of electrolyte-gated field-effect transistor (EGOFET), for ultrasensitive detection of the C-reactive protein (CRP). [...]

Cellulose nanocrystal interactions probed by thin film swelling to predict dispersibility

Publication year: 2016
Authors: Reid M.S. a, Villalobos M. b, Cranston E.D. *a
Published in: Nanoscale, 2016, Vol. 8, p. 12247-12257
The production of well-dispersed reinforced polymer nanocomposites has been limited due to poor understanding of the interactions between components. Measuring the cohesive particle–particle interactions and the adhesive particle–polymer interactions is challenging due to nanoscale dimensions and poor colloidal stability of nanoparticles in many solvents. [...]

On-line coupling of surface plasmon resonance optical sensing to size-exclusion chromatography for affinity assessment of antibody samples

Publication year: 2016
Authors: Dina Lakayan a, b, Rob Haselberg a, Wilfried M.A. Niessen a, c, Govert W. Somsen a, Jeroen Kool a
Published in: Journal of Chromatography A, Vol. 1452, p. 81–88
Surface plasmon resonance (SPR) is an optical technique that measures biomolecular interactions. Stand-alone SPR cannot distinguish different binding components present in one sample. Moreover, sample matrix components may show non-specific binding to the sensor surface [...]

Amine-appended polyaniline as a water dispersible electroactive polyelectrolyte and its integration into functional self-assembled multilayers

Publication year: 2016
Authors: Waldemar A. Marmisollé a, Eliana Mazaa b, Sergio Moya b, Omar Azzaroni a
Published in: Electrochimica Acta, 2016, Vol. 210, p. 435–444
We present an effective and simple method for the synthesis of poly(3-aminobenzylamine) (PABA) using a chemical oxidation strategy in aqueous solution. The polymer was characterized by NMR, LDI-TOF mass spectrometry, UV-visible, XPS and ATR-FTIR spectroscopies. [...]

Assembly of citrate gold nanoparticles on hydrophilic monolayers

Publication year: 2016
Authors: Vikholm-Lundin I. a, b, Rosqvist E. c, Ihalainen P. c, Munter T. d, Honkimaa A. e, Marjomäki V. f, Albers W.M. g, Peltonen J. c
Published in: Applied Surface Science, 2016, Vol. 378, p. 519–529
Self-assembled monolayers (SAMs) as model surfaces were linked onto planar gold films thorough lipoic acid or disulfide groups. The molecules used were polyethylene glycol (EG-S-S), N-[tris-(hydroxymethyl)methyl]acrylamide polymers with and without lipoic acid (Lipa-pTHMMAA and pTHMMAA) and a lipoic acid triazine derivative (Lipa-MF). [...]