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Early-stage detection of VE-cadherin during endothelial differentiation of human mesenchymal stem cells using SPR biosensor

Publication year: 2017
Authors: Fathi F. 1, Rezabakhsh A. 2, Rahbarghazi R. 3, Rashidi M.R. 4
Published in: Biosensors and Bioelectronics, 2017, Vol. 96, p. 358-366
Surface plasmon resonance (SPR) biosensors are most commonly applied for real-time dynamic analysis and measurement of interactions in bio-molecular studies and cell-surface analysis without the need for labeling processes. [...]

Interaction of Tissue Engineering Substrates with Serum Proteins and Its Influence on Human Primary Endothelial Cells

Publication year: 2017
Authors: Mohan T. 1, Niegelhell K. 2, Nagaraj C. 3,4, Reishofer D. 2,5, Spirk S. 2,5, Olschewski A. 3,4, Stana Kleinschek K. 5, Kargl R. 2,5
Published in: Biomacromolecules, 2017, Vol. 18 (2), p. 413–421
Polymer-based biomaterials particularly polycaprolactone (PCL) are one of the most promising substrates for tissue engineering.The surface chemistry of these materials plays a major role [...]

Surface Plasmon Resonance for Measuring Exocytosis from Populations of PC12 Cells: Mechanisms of Signal Formation and Assessment of Analytical Capabilities

Publication year: 2017
Authors: Moreira B. 1, Tuoriniemi J. 1, Kouchak Pour N. 1, Mihalčíková L.1, Safina G. 1,2
Published in: Analytical Chemistry, 2017, Vol. 89 (5), p. 3069–3077
Because of cell to cell variation, it is difficult to obtain statistically significant data on the frequency of exocytosis events (Rexocytosis, t–1 m–2) with traditional single cell electrophysiological [...]

Ionic strength-controlled hybridization and stability of hybrids of KRAS DNA single-nucleotides: A surface plasmon resonance study

Publication year: 2017
Authors: Giamblanco N. 1, Petralia S. 2, Conoci S. 3, Messineo C. 4, Marletta G. 4
Published in: Colloids and Surfaces B: Biointerfaces, 2017, Vol. 158, p. 41-46
The discrimination of a fully matched, unlabeled KRAS wild-type (WT) (C-G) target sample with respect to three of the most frequent KRAS codon mutations (G12 S (C-A), G12 R (C-C), G12C (C-T)) was investigated using an [...]

Improving the mechanical properties of CNF films by NMMO partial dissolution with hot calender activation

Publication year: 2017
Authors: Orelma H., Korpela A., Kunnari V., Harlin A., Suurnäkki A.
Published in: Cellulose 2017
Reinforcing of cellulose nanofibril (CNF) films by partial dissolution with N-methylmorpholine-N-oxide (NMMO) was investigated. The method investigated is composed of impregnation of CNF film [...]

Breast Cancer Targeting Peptide Binds Keratin 1: A New Molecular Marker for Targeted Drug Delivery to Breast Cancer

Publication year: 2017
Authors: Soudy R. 1,2,5, Etayash H. 1, Bahadorani K.1, Lavasanifar A. 1,3, Kaur K. 1,4
Published in: Molecular Pharmaceutics, 2017, Vol. 14 (3), p. 593–604
The biomarkers or receptors expressed on cancer cells and the targeting ligands with high binding affinity for biomarkers play a key role in early detection and treatment of breast cancer. [...]

Detection of transferrin by using a surface plasmon resonance sensor functionalized with a boronic acid monolayer

Publication year: 2017
Authors: Yanchao Mayang 1,2, Xiwen He 1,2, Langxing Chen 1,2, Yukui Zhang 1,2,3
Published in: Microchimica Acta, 2017, Vol. 184 (8), p. 2749–2757
A surface plasmon resonance sensor whose surface has been modified with boronic acid groups was developed in order to bind and detect transferrin. 4-Mercaptophenylboronic acid (4-MPBA) was utilized [...]

Dangerous liaisons: anion-induced protonation in phosphate-polyamine interactions and their implications for charge states of biologically relevant surfaces

Publication year: 2017
Authors: Laucirica G., Marmisollé W.A., Azzaroni O.
Published in: Physical Chemistry Chemical Physics, 2017, Vol. 19 (12), p. 8612-8620
Although not always considered a preponderant interaction, amine-phosphate interactions are omnipresent in multiple chemical and biological systems. [...]

Targeting Tumor-Associated Exosomes with Integrin-Binding Peptides

Publication year: 2017
Authors: Carney R.P. 1, Hazari S. 1, Rojalin T. 2,3, Knudson A. 1, Gao T. 3,4, Tang Y. 3,4, Liu R. 1, Viitala T. 5, Yliperttula M. 5,6, Lam K.S. 1,7
Published in: Advanced Biosystems, 2017, Vol. 1 (5), p. 1600038
All cells expel a variety of nanosized extracellular vesicles (EVs), including exosomes, with composition reflecting the cells' biological state. Cancer pathology is dramatically mediated by EV trafficking [...]

Integration of Biorecognition Elements on PEDOT Platforms through Supramolecular Interactions

Publication year: 2017
Authors: Sappia L.D. 1,2, Piccinini E. 3, Marmisollé W. 3, Santilli N. 1, Maza E. 4, Moya S. 4, Battaglini F. 5, Madrid R.E. 1,2, Azzaroni O. 3
Published in: Advanced Materials Interfaces, 2017, 1700502
The rapidly emerging field of organic bioelectronics exploits the functional versatility of conducting polymers to transduce biological recognition events into electronic signals. For the majority of biosensors [...]

Investigation of graphene-on-metal substrates for SPR-based sensor using finite-difference time domain

Publication year: 2017
Authors: Said F. A. 1, Menon P. S. 1, Rajendran V. 2, Shaari S. 1, Majlis B. Y. 1
Published in: IET Nanobiotechnology, 2017, Vol. 11, Issue 8, p. 981 – 986
In this study, the authors investigated the effects of a single layer graphene as a coating layer on top of metal thin films such as silver, gold, aluminum and copper using finite-difference time domain method. [...]

“Click” access to multilayer functionalized Au surface: A terpyridine patterning example

Publication year: 2017
Authors: F.A. Martin a, D. Marconi a, S. Neamtu a, T. Radu a, M. Florescu b, R. Turcu a, C. Lar c, N.D. Hădade c, I. Grosu c, I. Turcu a
Published in: Materials Science and Engineering: C, 2017, Vol. 75, p. 1343–1350
This work is focused on self-assembled monolayers (SAMs) fabrication, using two types of Au surfaces, by subsequent attachment of different layers in order to develop [...]

Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion

Publication year: 2017
Authors: Parveen N. 1, Block S. 1, Zhdanov V.P. 1,2, Rydell G.E. 3, Höök F. 1
Published in: Langmuir, 2017, Vol. 33 (16), p. 4049–4056
Multivalent receptor-mediated interactions between virions and a lipid membrane can be weakened using competitive nonpathogenic ligand binding. In particular, the subsequent binding [...]

Immuno-biosensor for Detection of CD20-Positive Cells Using Surface Plasmon Resonance

Publication year: 2017
Authors: Shanehbandi D. 1,2, Majidi J. 1, Kazemi T. 1,2, Baradaran B. 1, Aghebati-Maleki L. 1,2, Fathi F. 2,3, Ezzati Nazhad Dolatabadi J. 3
Published in: Advanced Pharmaceutical Bulletin, 2017, Vol. 7 (2), p.188-194
Purpose: Surface plasmon resonance (SPR) sensing confers a real-time assessment of molecular interactions between biomolecules and their ligands. This approach is highly sensitive [...]

Bovine Serum Albumin Adsorption at a Silica Surface Explored by Simulation and Experiment

Publication year: 2017
Authors: Kubiak-Ossovska K. 1, Tokarczyk K. 2, Jachimska B. 2, Mulheran A.P. 1
Published in: Journal of Physical Chemistry B, 2017, Vol. 121 (16), p. 3975–3986
Molecular details of BSA adsorption on a silica surface are revealed by fully atomistic molecular dynamics (MD) simulations (with a 0.5 μs trajectory), supported by dynamic light scattering (DLS), zeta potential, [...]

Angiogenic and Restorative Abilities of Human Mesenchymal Stem Cells Were Reduced Following Treatment With Serum From Diabetes Mellitus Type 2 Patients

Publication year: 2017
Authors: Rezaie J. 1, Mehranjani M.S. 1, Rahbarghazi R. 2,3, Shariatzadeh M.A. 1
Published in: Journal of Cellular Biochemistry, 2017, 9999, p. 1-12
This experiment investigated the impact of serum from patients with type 2 diabetes mellitus on the angiogenic behavior of human mesenchymal stem cells in vitro. Changes in the level [...]

Characterization of IFN-γ Detection and Electrochemical Proximity Assay by a Novel Multi-Parametric/Multimodal Spectroscopy Apparatus

Publication year: 2017
Authors: Zhou L., Chin B. A., Simonian A. L.*
Published in: ECS Transactions, 2017, Vol. 80, Issue 10, p. 1565-1570
Functional interfaces play a vital role in biosensing, which requires proper allocation, orientation, conformation, activities and stability to achieve high affinity and stability of the biomolecules. However, the characterization [...]

Layer-by-layer assemblies of highly connected polyelectrolyte capped-Pt nanoparticles for electrocatalysis of hydrogen evolution reaction

Publication year: 2017
Authors: Fenoy G.E. a, b, Maza E. a, c, Zelaya E. d, Marmisollé W.A. a, Azzaroni O. a
Published in: Applied Surface Science, 2017, Vol. 416, p. 24–32
Herein we present a simple one-step method to produce polyelectrolyte-capped Pt nanoparticles able to be assembled into layer-by-layer arrays [...]

Surface plasmon resonance and molecular docking studies of bovine serum albumin interaction with neomycin: kinetic and thermodynamic analysis

Publication year: 2017
Authors: Sharifi M. 1, Dolatabadi J.E.N. 2, Fathi F. 2,3, Zakariazadeh M. 4, Barzegar A. 4, Rashidi M. 2, Tajalli H. 1, Rashidi M.-R. 2
Published in: BioImpacts, 2017, Vol. 7(2), p. 91–97
Introduction: The interactions between biomacromolecules such as serum albumin (SA) and various drugs have attracted increasing research attention in recent years. [...]

Characterization of Covalently Bound Anti-Human Immunoglobulins on Self-Assembled Monolayer Modified Gold Electrodes

Publication year: 2017
Authors: Holzer B. 1, Manoli K. 1, Ditaranto N. 1,2, Macchia E. 1, Tiwari A. 1, Di Franco C. 3, Scamarcio G. 3,4, Palazzo G. 1,2, Torsi L. 1,2
Published in: Advanced Biosystems, 2017
Bioconjugated gold surfaces constitute interesting platforms for biosensing applications. The immobilization of antibodies such as anti-immunoglobulin G and M (anti-IgG and anti-IgM) [...]