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Handbook of MP-SPR

Publication year: 2019
Authors: Marquart A., Kuncová-Kallio J., Albers M., Bombera R., Ståhlberg R.
Published in: Handbook of MP-SPR, 2019, Vol. 1, Based on fifth edition of Arnoud Marquart's Surface Plasmon Resonance book
Handbook of Multi-Parametric Surface Plasmon Resonance for Molecular Interaction Analysis - Theory and Practice

Multilayer Density Analysis of Cellulose Thin Films

Publication year: 2019
Authors: Sampl C. 1,2ʈ, Niegelhell K. 1,2ʈ, Reishofer D. 1ʈ, Resel R. 3ʈ, Spirk S. 1,2*ʈ, Hirn U. 1,2ʈ
Published in: Biopolymer Thin Films and Coatings
An approach for the multilayer density analysis of polysaccharicle thin films at the example of cellulose is presented. In detail, a model was developed for the evaluation of the density in different layers across the thickness direction of the film.[...]

Self-assembled hybrid nanocomposite films of carbon dots and hydrolyzed collagen

Publication year: 2019
Authors: da Pinto T.S. a, Rodrigues P.N.S. a, Marinho L.E.S. a, Verly R.M. a, Bretas Roa J.P. 1, de Oliveira L.C.Z. b, Pereira F.V. b, de Magalhaes M.T.Q. c,d, de Mesquita J.P. a
Published in: Materials Chemistry and Physics, 2019, Vol. 230, p. 44-53
In the present work, hydrophilic carbon dots (CD) prepared by cotton cellulose were used in the building of self-assembled films with hydrolyzed collagen (CL) to verify [...]

Ch. 11: Optical and Acoustic Label-free Instrumentation for Molecular Detection with a Focus on Food

Publication year: 2019
Authors: J. Kuncová-Kallio, S. Auer, A. Spehar, H.H. Qu, D. Spasic, J. Lammertyn
Published in: Rapid Antibody-based technologies is Food Analysis, 2019, Vol. 15, p. 223-255
Food Chemistry, Function and Analysis: Optical and Acoustic Label-free Instrumentation for Molecular Detection with a Focus on Food

Use of a Branched Linker for Enhanced Biosensing Properties in IgG Detection from Mixed Chinese Hamster Ovary Cell Cultures

Publication year: 2019
Authors: Islam N. a,b, Gurgel P.V. a,c, Rojas O.J. a,d, Carbonell R.G. a,e
Published in: Bioconjugate Chemistry, 2019, Vol. 30, p. 815−825
Tris(2-aminoethyl)-amine (TREN), a branched amine, was coupled to planar surfaces of alkanethiol self-assembled monolayers (SAMs) to increase [...]

Refractive Index and Sensing of Glucose Molarities Determined using Au-Cr K-SPR at 670/785 nm Wavelenght

Publication year: 2019
Authors: P. Susthitha Menon*, Budi Mulyanti, Nur Akmar Jamil, Chandra Wulandari, Harbi Setyo Nugroho, Gan Siew Mei, Noor Faizah Zainul Abidin, Lilik Hasanah, Roer Eka Pawinanto & Dilla Duryha Berhanuddin
Published in: Sains Malaysiana 48(6)(2019): 1259–1265

Enzyme Immobilization in Polyelectrolyte Brushes: High Loading and Enhanced Activity Compared to Monolayers

Publication year: 2019
Authors: del Castillo G.F-D. a, Koenig M. b, Mueller M. b, d, Eichhorn K.-J. b, Stamm M. b,d, Uhlmann P. b,c, Dahlin A. a
Published in: Langmuir, 2019, Vol. 35, p. 3479-3489
Catalysis by enzymes on surfaces has many applications. However, strategies for efficient enzyme immobilization with preserved activity are still in need of further development. [...]

Trypanosoma cruzi Virulence Factors for the Diagnosis of Chagas’ Disease

Publication year: 2019
Authors: Cecilia Yamil Chain,*,† Dênio Emanuel Pires Souto,*,‡ María Laura Sbaraglini,§ Carlos A. Labriola,∥ María Antonieta Daza Millone,† Eduardo Alejandro Ramirez,† José Sebastián Cisneros,† Constanza Lopez-Albizu,⊥ Karenina Scollo,⊥ Lauro T. Kubota,# Andrés Mariano Ruiz,⊥ and María Elena Vela†
Published in: ACS Infectious Diseases
trans-Sialidase and cruzipain are important virulence factors from Trypanosoma cruzi, the etiological agent of Chagas disease, that have highly antigenic domains in their structure and were reported as potential tools for diagnosis of the illness. [...]

Maleic anhydride and acetylene plasma copolymer surfaces for SPR immunosensing

Publication year: 2019
Authors: Makhneva E. 1,2, Farka Z. 3, Pastucha M. 4,5, Obrusník A. 1,2, Horáčková V. 4, Skládal P. 4,5, Zajíčková L. 1,2
Published in: Analytical and Bioanalytical Chemisry, 2019
We report on the successful application of carboxyl-rich plasma polymerized (PP) films as a matrix layer for bioreceptor immobilization in surface plasmon resonance (SPR) immunosensing. Composition and [...]

Label-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules

Publication year: 2019
Authors: Tasbasi BB 1, Guner BC 1, Sudagidan M 1, Ucak S 2, Kavruk M3, Ozalp VC 4.
Published in: Analytical Biochemistry
Lateral flow assay (LFA) type of biosensors have been popular due to cost-effectiveness and easy-interpretation for instant results, most common examples of applications being pregnancy tests, food safety or medical diagnostics. There are several examples of reports with high sensitivity, including pre-concentration of the sample by magnetic pull-down. However, sensitivity and direct detection designs with aptamers has been a limiting factor for developing aptamers-based LFA assays. [...]

Layer‐by‐Layer Formation of Polyamine‐Salt Aggregate/Polyelectrolyte Multilayers. Loading and Controlled Release of Probe Molecules from Self‐Assembled Supramolecular Networks

Publication year: 2019
Authors: Herrera S.E. 1, Agazzi M.L. 1, Cortez M.L. 1, Marmisollé W.A. 1, von Bildering C. 1,2, Azzaroni O. 1
Published in: Macromolecular Chemistry and Physics, 2019
The use of soft materials as building blocks in layer‐by‐layer (LbL) assemblies represent a very appealing and useful approach to enhance the loading capacity of thin films. Here, the capacity of positively charged polyamine‐salt aggregates (PSAs) [...]

A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders

Publication year: 2019
Authors: Ariana de Souza Moraes 1,2,3, Doralina Guimarães Brum3,4, Jéssica Cristiane Magalhães Ierich 1,2,3, Akemi Martins Higa1,2,3, Amanda Stefanie Jabur Assis2,3, Celina Massumi Miyazaki2, Flávio Makoto Shimizu5, Luís Antonio Peroni 6, M. Teresa Machini7, Amilton Antunes Barreira8,9, Marystela Ferreira 2, Osvaldo N. Oliveira Jr.5 & Fabio Lima Leite2,3
Published in: Scientific Reports
A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients’ prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). [...]

Self-assembled hybrid nanocomposite films of carbon dots and hydrolyzed collagen

Publication year: 2019
Authors: Tarciane da S. Pinto a, Priscilla N.S. Rodrigues a, Luiz E.S. Marinho a, Rodrigo M. Verly a, J.P. Bretas Roa a, Luiz C.A. de Oliveira b, Fabiano V. Pereira b, Mariana T.Q. de Magalhães c,d, João P. de Mesquita a
Published in: Materials Chemistry and Physics, 2019, Vol. 230, p. 44-53
In the present work, hydrophilic carbon dots (CD) prepared by cotton cellulose were used in the building of self-assembled films with hydrolyzed collagen (CL) to verify their properties, especially molecular interactions and morphology. [...]

Synergy between Zwitterionic Polymers and Hyaluronic Acid Enhances Antifouling Performance

Publication year: 2019
Authors: Yinqiang Xia,† Vahid Adibnia,‡ Cancan Shan,† Renliang Huang,§ Wei Qi,†,# Zhimin He,† Guojun Xie,∥ Mateusz Olszewski,∥ Gregory De Crescenzo,⊥ Krzysztof Matyjaszewski,*,∥ Xavier Banquy,*,‡ and Rongxin Su*,†,#
Published in: Langmuir
Challenges associated with nonspecific adsorption of proteins on sensor surfaces have steered the development of novel antifouling materials and strategies. Inspired by human synovial fluid composition and structure, we designed synergistic antifouling coatings with mixtures of hyaluronic acid (HA) and a zwitterionic bottlebrush polymer (BB). [...]

Real-Time Label-Free Targeting Assessment and in Vitro Characterization of Curcumin-Loaded Poly-lactic-co-glycolic Acid Nanoparticles for Oral Colon Targeting

Publication year: 2019
Authors: Mohamed A. Akl,†,‡ Alma Kartal-Hodzic,† Teemu Suutari,† Timo Oksanen,† Isabella Monia Montagner,§ Antonio Rosato,§,∥ Hatem R. Ismael,‡ Mohsen I. Afouna,‡ Paolo Caliceti,⊥ Marjo Yliperttula,†,⊥ Ahmed M. Samy,‡ Francesca Mastrotto,⊥ Stefano Salmaso,⊥ and Tapani Viitala*,†,#
Published in: ASC Omega
The exploitation of curcumin for oral disease treatment is limited by its low solubility, poor bioavailability, and low stability. Surface-functionalized poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) have shown promising results to ameliorate selective delivery of drugs to the gastrointestinal tract. [...]

Kinetic and thermodynamic insights into interaction of albumin with piperacillin: Spectroscopic and molecular modeling approaches

Publication year: 2019
Authors: Farzaneh Fathi a, Maryam Sharifi b, c, d, Amir Jafari e, Naser Kakavandi f, Soheila Kashanian g, h, Jafar Ezzati Nazhad Dolatabadi c, *, Mohammad-Reza Rashidi c, i, **
Published in: Journal of Molecular Liquids
The current study investigated the binding of BSA and piperacillin at multifarious concentrations (1 e128 mM) along with four different temperatures. To do this, three sensitive methods were employed including surface plasmon resonance (SPR), fluorescence spectroscopy and molecular docking (MD). [...]

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation

Publication year: 2019
Authors: Antonella Managò1,12, Valentina Audrito1,12, Francesca Mazzola2, Leonardo Sorci 3, Federica Gaudino1, Katiuscia Gizzi4, Nicoletta Vitale5, Danny Incarnato6, Gabriele Minazzato7, Alice Ianniello8, Antonio Varriale9, Sabato D’Auria9, Giulio Mengozzi8, Gianfranco Politano 10, Salvatore Oliviero 4,11, Nadia Raffaelli7,13 & Silvia Deaglio 1,13
Published in: Nature communications
Damage-associated molecular patterns (DAMPs) are molecules that can be actively or passively released by injured tissues and that activate the immune system. Here we show that nicotinate phosphoribosyltransferase (NAPRT), detected by antibody-mediated assays and mass spectrometry, is an extracellular ligand for Toll-like receptor 4 (TLR4) and a critical mediator of inflammation, acting as a DAMP. Exposure of human and mouse macrophages to NAPRT activates the inflammasome and NF-κB for secretion of inflammatory cytokines. [...]

ORP2 interacts with phosphoinositides and controls the subcellular distribution of cholesterol

Publication year: 2019
Authors: Koponen A. a, Arora A. a, Takahashi K. b, Kentala H. a, Kivelä A.M. a, Jääskeläinen E. a, Peränen J. b, Somerharju P. c, Ikonen E. b, Viitala T. d, Olkkonen V.M. a,b
Published in: Biochimie, 2019, Vol. 158, p. 90-101
ORP2 is a sterol-binding protein with documented functions in lipid and glucose metabolism, Akt signaling, steroidogenesis, cell adhesion, migration and proliferation. [...]

Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials

Publication year: 2019
Authors: Sarah Dedisch 1,2 Annika Wiens 2 Mehdi D. Davari 2 Dominik Söder 1 Cesar Rodriguez‐Emmenegger 1,3 Felix Jakob 1,2 Ulrich Schwaneberg 1,2
Published in: Biotechnology and Bioengineerging
Enzyme immobilization is extensively studied to improve enzyme properties in catalysis and analytical applications. Here, we introduce a simple and versatile enzyme immobilization platform based on adhesion‐promoting peptides, namely Matter‐tags. [...]

Development of a surface plasmon resonance sensor for coupling to capillary electrophoresis allowing affinity assessment of protein mixture components

Publication year: 2018
Authors: Domínguez-Vega E. a, Haselberg R. a, van Iperen D. b, Kool J. a, Somsen G.W. a, de Jong G.J. c
Published in: Sensors and Actuators B: Chemical, 2018, Vol. 254, p. 1040-1047
Surface plasmon resonance (SPR) currently is the major platform to study protein–protein interactions, but it lacks the selectivity o distinguish between binding components within one sample. [...]