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The effect of coatings on the performance of the microelectrode arrays

Publication year: 2014
Authors: Sivasubramaniapandian Manoj
Published in: Master's Thesis
Microelectrode arrays (MEAs) are pretreated with suitable surface molecules prior to culturing of cells, since the properties of the substrate have a decisive effect on the adhesion, migration, differentiation, and the growth of the cells. The purpose of this thesis is to study a few of these coating materials, and to evaluate their impact on the performance of the MEA. [...]

An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes

Publication year: 2014
Authors: Ihalainen P. 1, Pettersson F. 2, Pesonen M. 2, Viitala T. 3, Määttänen A.1, Österbacka R. 2, Peltonen J. 1
Published in: Nanotechnology, 2014, Vol. 25, Issue 9
In this study, two different supramolecular recognition architectures for impedimetric detection of DNA hybridization have been formed on disposable paper-supported inkjet-printed gold electrodes. The gold electrodes were fabricated using a gold nanoparticle based ink. The first recognition architecture consists of subsequent layers of biotinylated self-assembly monolayer (SAM), streptavidin and biotinylated DNA probe. [...]

A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations

Publication year: 2014
Authors: Tang W., Hu S., Wang H., Zhao Y., Li N., Liu F.*
Published in: Chemical Communications, 2014, Vol. 50, Issue 92, p. 14352-14355
A universal molecular translator based on the target-triggered DNA strand displacement was developed, which was able to convert various kinds of non-nucleic acid targets into a unique output DNA. [...]

Dynamic and equilibrium performance of sensors based on short peptide ligands for affinity adsorption of human IgG using surface plasmon resonance

Publication year: 2014
Authors: Islam N. 1, Shen F. 1, Gurgel P.V. 1, Rojas O.J. 2, Carbonell R.G. 3
Published in: Biosensors and Bioelectronics, Vol. 58, 2014, p. 380-387
This paper characterizes the potential of novel hexameric peptide ligands for on-line IgG detection in bioprocesses. Surface Plasmon Resonance (SPR) was used to study the binding of human IgG to the hexameric peptide ligand HWRGWV, which was covalently grafted to alkanethiol self-assembled monolayers (SAM) on gold surfaces. [...]

Effect of gold nanoparticles on the structure and electron-transfer characteristics of glucose oxidase redox polyelectrolyte-surfactant complexes

Publication year: 2014
Authors: Cortez M.L. a,b, Marmisollé W. a, Pallarola D. b, Pietrasanta L.I. c, Murgida D.H. a, Ceolín M. b, Azzaroni O. b, Battaglini F. a
Published in: Chemistry A European Journal, 2014, Vol. 20, Issue 41, p. 13037–13404
Efficient electrical communication between redox proteins and electrodes is a critical issue in the operation and development of amperometric biosensors. The present study explores the advantages of a nanostructured redox-active polyelectrolyte-surfactant complex containing [Os(bpy)2Clpy](2+) (bpy=2,2'-bipyridine, py= pyridine) as the redox centers and gold nanoparticles (AuNPs) as nanodomains for boosting the electron-transfer propagation throughout the assembled film in the presence of glucose oxidase (GOx). [...]

Modification of Cellulose Nanofibrils with Luminescent Carbon Dots

Publication year: 2014
Authors: Junkka K. 1, Guo J. 1, Filpponen I. 1, Lainen J. 1, Rojas O.J. 1,2
Published in: Biomacromolecules, 2014, Vol. 15 (3), p. 876–881
Films and hydrogels consisting of cellulose nanofibrils (CNF) were modified by covalent EDC/NHS coupling of luminescent, water-dispersible carbon dots (CDs). [...]

Triggering Protein Adsorption on Tailored Cationic Cellulose Surfaces

Publication year: 2014
Authors: Mohan T. 1, Niegelhell K. 4, Pinto Zarth C.S. 2, Kargl R. 3, Köstler S. 5, Ribitsch V. 1, Heinze T. 2, Spirk S. 1,4, Stana-Kleinschek K. 3
Published in: Biomacromolecules, 2014, Vol. 15 (11), p. 3931–3941
The equipment of cellulose ultrathin films with BSA (bovine serum albumin) via cationization of the surface by tailor-made cationic celluloses is described. In this way, matrices for controlled protein deposition are created, whereas the extent of protein affinity to these surfaces is controlled by the charge density and solubility of the tailored cationic cellulose derivative. [...]

Surface Plasmon Resonance in Living Cell Sensing

Publication year: 2014
Authors: Teemu Suutari
Published in: Master's Thesis
Surface plasmon resonance (SPR) is a label free technique to study surface interactions. It is based on photon-plasmon coupling. Laser light is directed through a prism and reflects form a metal surface, often gold. At certain conditions, photons turn into plasmons, which then propagate [...]

Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor

Publication year: 2013
Authors: Chang Zeng (a), Xin Huang (a), Jiangmin Xu (a), Guifen Li (a), Jie Ma (a), Hai-Feng Ji (b), Shuifang Zhu (a), Hongjun Chen (a)
Published in: Analytical Biochemistry, Vol. 440 (2013), p. 18–22
We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maizechlorotic mottle virus (MCMV). [...]

Water-Wettable Polypropylene Fibers by Facile Surface Treatment Based on Soy Proteins

Publication year: 2013
Authors: Salas C. 1,3, Genzer J. 2, Lucia L.A 1, Hubbe A.M. 1, Rojas O.J. 1,2, 4
Published in: ACS Applied Materials & Interfaces, 2013, Vol. 5, p. 6541 − 6548
Modification of the wetting behavior of hydrophobic surfaces is essential in a variety of materials, including textiles and membranes that require control of fluid interactions, adhesion, transport processes, sensing, etc. This investigation examines the enhancement of wettability of animportant class of textile materials, viz., polypropylene (PP) fibers, by surface adsorption of different proteins from soybeans, including soyflour, isolate, glycinin, and β-conglycinin. [...]

Self-assembly of pyridine-modified lipoic Acid derivatives on gold and their interaction with thyroxine (t4)

Publication year: 2013
Authors: Albers WM, Milani R, Tappura K, Munter T, Resnati G, Metrangolo P.
Published in: International Journal of Molecular Sciences, 2013 Feb 6;14(2):3500-13.
Pyridyl derivatives of lipoic acid were prepared as ligands for the study of the interaction with thyroxine (T4). Thin self-assembled films of the ligands were prepared in 70% ethanol on gold and their interaction with T4 was studied by titration experiments in an aqueous buffer solution using Surface Plasmon Resonance (SPR). [...]

Non-labelled surface sensitive techniques as platforms for pharmaceutical nanotechnology research

Publication year: 2013
Authors: Huamin Liang, 2013
Published in: Doctor's Dissertation (article-based)
Insufficient delivery of drugs to the target sites like tumors and cells has been a barrier for achieving satisfying therapeutic effects in many diseases. Distribution and exposure of drugs to normal and healthy tissues may enhance the possibility of side effects and toxicity in vivo. Nanoparticle (NP) drug delivery systems have been developed to enable targeting of drugs to target sites and at the same time also reduce or even eliminate the distribution and exposure of drugs to non-targeted sites (normal and healthy tissues). The interactions of ligand attached NPs with specific receptors on the cell surface enable intracellular delivery of drugs. [...]

Elucidating the Signal Responses of Multi-Parametric Surface Plasmon Resonance Living Cell Sensing: A Comparison between Optical Modeling and Drug–MDCKII Cell Interaction Measurements

Publication year: 2013
Authors: Tapani Viitala 1, Niko Granqvist 1, Susanna Hallila 1, Marjo Yliperttula 1, Manuela Raviña 2
Published in: PLoS ONE, 2013, Vol. 8(8), e72192
In vitro cell-based assays are widely used during the drug discovery and development process to test the biological activity of new drugs. Most of the commonly used cell-based assays, however, lack the ability to measure in real-time or under dynamic conditions (e.g. constant flow). In this study a multi-parameter surface plasmon resonance approach in combination with living cell sensing has been utilized for monitoring drug-cell interactions in real-time, under constant flow and without labels. [...]

Self-Defensive Biomaterial Coating Against Bacteria and Yeasts: Polysaccharide Multilayer Film with Embedded Antimicrobial Peptide

Publication year: 2013
Authors: Dr. G. Cado, L. Séon, Dr. T. Garnier, Dr. A. Parat,Dr. Y. Frère, Dr. L. Jierry, Prof. P. Schaaf, Dr. F. Boulmedais (1); R. Aslam, Dr. R. Fabre, A. Chassepot, Dr. J.-C. Voegel,Dr. B. Senger, Prof. P. Schaaf, Dr M.-H. Metz-Boutigue (2); Prof. F. Schneider (3); Dr. H. Kerdjoudj (4); Dr. L. Jierry, Prof. P. Schaaf, Dr. F. Boulmedais (5); Prof. P. Schaaf (6)
Published in: Advanced Functional Materials, 2013
Prevention of pathogen colonization of medical implants is a major medicaland fi nancial issue since infection by microorganisms constitutes one of the most serious complications after surgery or critical care. Immobilization ofantimicrobial molecules on biomaterials surfaces is an efficient approach to prevent biofilm formation. Herein, the first self-defensive coating against both bacteria and yeasts is reported where the release of the antimicrobial peptide is triggered by enzymatic degradation of the film due to the pathogens themselves. [...]

Label-Enhanced Surface Plasmon Resonance: A New Concept for Improved Performance in Optical Biosensor Analysis

Publication year: 2013
Authors: Niko Granqvist 1,2, Anders Hanning 3, Lars Eng 3, Jussi Tuppurainen 2, Tapani Viitala 1
Published in: Sensors, 2013, Vol. 13(11), p. 15348-15363
Surface plasmon resonance (SPR) is a well-established optical biosensor technology with many proven applications in the study of molecular interactions as well as in surface and material science. SPR is usually applied [...]

Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility

Publication year: 2013
Authors: H. G. Wang (1,2), T. Y. Yin (1,2), S. P. Ge (1,2), Q. Zhang (1,2), Q. L. Dong (1,2), D. X. Lei (1,2), D. M. Sun (1,2), G. X. Wang (1,2)
Published in: Journal of Biomedical Materials Research Part A, 2013, Vol. 101A, p. 413–420
In-stent restenosis and subsequent thrombosis remain a significant complication following the implantation of coronary stents. Different approaches have been used in developing novel coronary stents to protect against thrombosis and minimize restenosis. In the present study, we designed a biomacromolecular layer-by-layer coating with heparin, vascular endothelial growth factor (VEGF), and bronectin onto nickel-free titanium surface to improve blood compatibility and endothelial cell proliferation. [...]

Specificity and regenerability of short peptide ligands supported on polymer layers for immunoglobulin G binding and detection

Publication year: 2013
Authors: Zhang Y. 1, Islam N. 2, Carbonell R.G. 2, Rojas O.J. 1,2
Published in: ACS Applied Materials and Interfaces, 2013, Vol.5 (16), p. 8030-7
We demonstrate the specificity, regenerability, and excellent storage stability of short peptide-based systems for detection of immunoglobulin G (IgG). The bioactive component consisted of [...]

Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi

Publication year: 2013
Authors: D'Angelo G. 1, Uemura T., Chuang C.C., Polishchuk E., Santoro M., Ohvo-Rekilä H., Sato T., Di Tullio G., Varriale A., D'Auria S., Daniele T., Capuani F., Johannes L., Mattjus P., Monti M., Pucci P., Williams R.L., Burke J.E., Platt F.M., Harada A., De Matteis M.A.
Published in: Nature Research Letter, 2013, Vol. 501, p. 7465
Newly synthesized proteins and lipids are transported across the Golgi complex via different mechanisms whose respective roles are not completely clear. We previously identified a non-vesicular intra-Golgi transport pathway for glucosylceramide (GlcCer)—the common precursor of the different series of glycosphingolipids—that isoperated by the cytosolic GlcCer-transfer protein FAPP2 (also known as PLEKHA8). [...]

2NH and 3OH are crucial structural requirements in sphingomyelin for sticholysin II binding and pore formation in bilayer membranes

Publication year: 2013
Authors: Terhi Maula (a), Y. Jenny E. Isaksson (a), Sara García-Linares (b), Sanna Niinivehmas (c), Olli T. Pentikäinen (c),Mayuko Kurita (d), Shou Yamaguchi (d), Tetsuya Yamamoto (d), Shigeo Katsumura (d), José G. Gavilanes (b), Álvaro Martínez-del-Pozo (b), J. Peter Slotte (a)
Published in: T. Maula et al. Biochimica et Biophysica Acta, Vol. 1828 (2013), p. 1390–1395
Sticholysin II (StnII) is a pore-forming toxin from the sea anemone Stichodactyla heliantus which belongs to the large actinoporin family. The toxin binds to sphingomyelin (SM) containing membranes, and shows high binding speci?city for this lipid. In this study, we have examined the role of the hydrogen bondinggroups of the SM long-chain base (i.e., the 2NH and the 3OH) for StnII recognition. [...]

Specific water uptake of thin films from nanofibrillar cellulose

Publication year: 2013
Authors: Katri S. Kontturi (a), Eero Kontturi (a), Janne Laine (a)
Published in: J.Mater. Chem. A, 2013
Swelling of NFC films was surveyed by quartz crystal microbalance with dissipation monitoring coupled with H2O/D2O exchange and surface plasmon resonance. The influence of charged groups on swelling was investigated by neutralizing the charge of NFC with cationic polyelectrolytes or increasing the charge with carboxymethyl cellulose (CMC). [...]