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Fluid dynamics modeling for synchronizing surface plasmon resonance and quartz crystal microbalance as tools for biomolecular and targeted drug delivery studies

Publication year: 2012
Authors: Tapani Viitala (a); Huamin Liang (a); Mayur Gupta (a), (b); Thomas Zwinger (c); Marjo Yliperttula (a); Alex Bunker (d); (e); ⇑
Published in: Journal of Colloid and Interface Science, Vol. 378 (2012), p. 251–259
We have used computational fluid dynamics modeling (CFD) to synchronize the flow conditions in the flow channels of two complementary surface-sensitive characterization techniques: surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). Since the footprint of the flow channels of the two devices is specified by their function, the flow behavior can only be varied either by altering the height ofthe flow channel, or altering the volumetric rate of flow (flow rate) through the channel. [...]

Real-Time Label-Free Monitoring of Drug- or Nanoparticle-Cell Interactions

Publication year: 2012
Authors: Tapani Viitala, Niko Granqvist, Huamin Liang, Marjo Yliperttula
Published in: Newsletter of the Controlled Release Society, 2012, Vol. 29, No. 6, p. 14-16
Efficient drug delivery is one of the major challenges in modern pharmaceutical research. There is a constant need for developing new formulation strategies for new drug delivery systems because new chemical entities tend to have low solubility, and biotechnology drugs (e.g., DNA, oligonucleotides, antibodies, and proteins) permeate poorly to the target sites, limiting or even prohibiting their therapeutic use. [...]

Structural and Functional Characteristics of Chimeric Avidins Physically Adsorbed onto Functionalized Polythiophene Thin Films, Albers et al. ACS Applied Materials & Interfaces

Publication year: 2012
Authors: Willem M. Albers †, Jani M. Pelto †, Clèment Suspenè ‡, Juha A.Määttä §, Abderrahim Yassar ⊥, Vesa P. Hytönen §#, Inger M.Vikholm-Lundin †, Kirsi Tappura †
Published in: ACS Applied Materials and Interfaces, 2012, Vol. 4, p. 4067 − 4077
Stabilized bioreceptor layers are of great importance in the design of novel biosensors. In earlier work, chimeric avidins enabled immobilization of biotinylated anti-bodies onto gold surfaces with greater stability compared to more conventional avidins (wild-type avidin and streptavidin). [...]

Adsorption of PEO-PPO-PEO Triblock Copolymers with End-Capped Cationic Chains of Poly(2-dimethylaminoethyl methacrylate)

Publication year: 2011
Authors: Xiaomeng Liu†, Arja-Helena Vesterinen§, Jan Genzer‡, Jukka V. Seppälä§, and Orlando J. Rojas*†∥
Published in: Langmuir, 2011, Vol. 27 (16), p. 9769–9780
We study the adsorption of a symmetric triblockcopolymer of ethylene oxide, EO, and propylene oxide, PO,end-capped with quarternized poly(2-dimethylaminoethylmethacrylate), DMAEMA (DMAEMA24EO132PO50EO132DMAEMA24). Light scattering and tensiometry are used tomeasure the relative size of the associated structures and surfaceexcess at the airliquid interface. [...]

Surface plasmon resonance biosensor with high anti-fouling ability for the detection of cardiac marker troponin T

Publication year: 2011
Authors: J.T. Liu a, C.J. Chen b, T. Ikoma c, T. Yoshioka c, J.S. Cross d,e, S.-J.Chang a, J.-Z. Tsai b, J. Tanaka c
Published in: Analytica Chimica Acta, 2011, Vol. 703(1), p. 80-86
Designing a surface recognition layer with high anti-fouling ability, high affinity, and high specificity is an important issue to produce high sensitivity biosensing transducers. In this study, a self-assembled monolayer (SAM) consisting of a homogeneous mixture of oligo(ethylene glycol) (OEG)-terminated alkanethiolate and mercaptohexadecanoic acid (MHDA) on Au was employed for immobilizingtroponin T antibody and applied in detecting cardiac troponin T by using surface plasmon resonance (SPR). [...]

Modification of cellulose films by adsorption of CMC and chitosan for controlled attachment of biomolecules

Publication year: 2011
Authors: Hannes Orelma 1, Ilari Filpponen 2, Leena-Sisko Johansson 3, Janne Laine 4, Orlando Jose Rojas 5
Published in: Biomacromolecules, 2011, Vol. 12 (12), p. 4311–4318
The adsorption of human immunoglobulin G (hIgG) and bovine serum albumin (BSA) on cellulose supports were investigated. The dynamics and extent of related adsorption processes were monitored by surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D). Amine groups were installed on the cellulose substrate by adsorption of chitosan from aqueous solution, which allowed for hIgG to physisorb from acid media and produced a functionalized substrate with high surface density (10 mg/m2). [...]

Development of diagnostic SPR based biosensor for the detection of pharmaceutical compounds in saliva

Publication year: 2010
Authors: Susanna Sonny, Adama M. Sesay, Vesa Virtanen
Published in: Proc. SPIE 7376, Laser Applications in Life Sciences, 737605
The aim of the study is to develop diagnostic tests for the detection of pharmaceutical compounds in saliva. Oral fluid is increasingly being considered as an ideal sample matrix. It can be collected non-invasively and causes less stress to the person being tested. [...]

Adsorption of a Nonionic Symmetric Triblock Copolymer on Surfaces with Different Hydrophobicity

Publication year: 2010
Authors: Xiaomeng Liu†, Dong Wu‡, Salomon Turgman-Cohen§, Jan Genzer§, Thomas W. Theyson∥ and Orlando J. Rojas*†⊥∥
Published in: Langmuir 2010, 26(12), 9565–9574
This study investigates the adsorption of a symmetric triblock nonionic polymer comprising ethylene oxide (EO) and propylene oxide (PO) blocks (Pluronic P-105, EO37PO56EO37) on a range of substrates including hydrophobic, i.e., polypropylene (PP), poly(ethylene terephthalate) (PET), nylon, and graphite, and hydrophilic, i.e., cellulose and silica.[...]

The Development of a diagnostic test for the detection of drugs in saliva using a disposable sample preparation Microfluidic cartridge

Publication year: 2010
Authors: Sesay A M.1, Krühne U.2, Sonny S.3, Lund Olesen T.4, Virtanen V.5
Published in: µTAS 2010 Groningen (2010), Verpoorte, S. et al.(Eds.), 1520-1522pp ISBN 978-0-9798064-3-8
In this paper we report on the development of a diagnostic test for the detection of illegal drugs in saliva [...]

Affinity Interaction between Hexamer Peptide Ligand HWRGWV and Immunoglobulin G Studied by Quartz Crystal Microbalance and Surface Plasmon Resonance

Publication year: 2010
Authors: Fei Sen; Under the direction of Dr. Ruben G. Carbonell
Published in: Ph.D Dissertation, 2010, Chemical Engineering
SHEN, FEI. Affinity Interaction between Hexamer Peptide Ligand HWRGWV and Immunoglobulin G Studied by Quartz Crystal Microbalance and Surface Plasmon Resonance. (Under the direction of Dr. Ruben G. Carbonell.) Immunoglobulins (Ig), also referred to as antibodies, act as protective agents against pathogens trying to invade an organism. Human immunoglobulin G (hIgG), as the most prominent immunoglobulin presented in serum and other human fluids, has broad applications in fields like immunotherapy and clinical diagnostics. [...]

Measurement of phthalocyanine binding kinetics with surface plasmon resonance method

Publication year: 2010
Authors: M. Karjalainen, V. Chukharev, N.V. Tkachenko, J. Lekkala
Published in: Poster, presented at Annual Meeting of European Optical Society EOS. October 26 -29, 2010, Paris, France
Phthalocyanine molecules are potential candidates for the structural elements of optoelectronic devices such as organic solar cells and chemical sensors. The binding self-assembling monolayers of phthalocyanine molecules to a gold surface was studied using surface plasmon resonance (SPR) method. [...]

Surface Plasmon Resonance on Nanoscale Organic Films

Publication year: 2010
Authors: Willem M. Albers and Inger Vikholm-Lundin
Published in: Nano-Bio-Sensing, 1st ed, Chapter 4, Springer 2010
Surface Plasmon Resonance (SPR) has evolved over the past 20 years into a very versatile detection method, particularly in biosci- ence applications. Not only the scientific literature [...]

Surface plasmon resonance instrument as a refractometer for liquids and ultrathin films

Publication year: 2010
Authors: H.Liang a,b, H.Miranto b,c, N.Granqvist b,d, J.W. Sadowski c, T.Viitala b, B.Wang a, M.Yliperttula b
Published in: Sensors and Actuators B: Chemical 2010, 149(1), 212-220
A surface plasmon resonance (SPR) setup in Kretschmann configuration is being utilized as a refractometer for both liquids as well as ultrathin films. The SPR signal detection technology used is based on a goniometer approach providing a wide angular scan range which facilitates highly accurate liquid and gas phase measurements. [...]

Photo-Electroactive Organic Nanosystems

Authors: V. Chukharev, N. Tkatchenko, J. Lekkala
Published in: Poster: Department of Chemistry and Bioengineering and Department of Automation Science and Engineering,Tampere University of Technology, Finland; Shubnikov Institute of Crystallography, Russian Academy of Sciences, Moscow
Project was focused on testing photo-electroactive molecules for solar and sensor technologies. Phthatalyanines, porphyrins and other organic molecules where characterized with Surface Plasmon Resonance (SPR), Atomic Force Microscope (AFM), and X-ray crystallography. [...]