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Hybrid POSS-Containing Brush on Gold Surfaces for Protein Resistance

Publication year: 2013
Authors: Xiaolu Ye (a), Junbo Gong (b), Zhongping Wang (b), Zengming Zhang (b), Sheng Han (c), Xuesong Jiang (a)*
Published in: Macromol. Biosci. 2013, 13, 921–926
A hybrid polymer brush containing poly(ethylene glycol) (PEG) chains and polyhedraloligosilsesquioxane (POSS) on a gold surface is presented that exhibits an excellent protein resistance and long-term stability. [...]

Elucidating the Signal Responses of Multi-Parametric Surface Plasmon Resonance Living Cell Sensing: A Comparison between Optical Modeling and Drug–MDCKII Cell Interaction Measurements

Publication year: 2013
Authors: Tapani Viitala 1, Niko Granqvist 1, Susanna Hallila 1, Marjo Yliperttula 1, Manuela Raviña 2
Published in: PLoS ONE, 2013, Vol. 8(8), e72192
In vitro cell-based assays are widely used during the drug discovery and development process to test the biological activity of new drugs. Most of the commonly used cell-based assays, however, lack the ability to measure in real-time or under dynamic conditions (e.g. constant flow). In this study a multi-parameter surface plasmon resonance approach in combination with living cell sensing has been utilized for monitoring drug-cell interactions in real-time, under constant flow and without labels. [...]

Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development

Publication year: 2013
Authors: Ihalainen P. 1, Majumdar H. 2,†, Viitala T. 3, Törngren B. 1, Närjeoja T. 4, Määttänen A. 1, Sarfraz J. 1, Härmä H. 4, Yliperttula M. 3, Österbacka R. 2, Peltonen J. 1
Published in: Biosensors, 2013, Vol. 3, p. 1-17
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRPantibody are grown on printed gold electrodes on disposable paper-substrates. [...]

Characterizing Ultrathin and Thick Organic Layers by Surface Plasmon Resonance Three-Wavelength and Waveguide Mode Analysis

Publication year: 2013
Authors: Niko Granqvist †, Huamin Liang †, Terhi Laurila †, Janusz Sadowski ‡, Marjo Yliperttula †, and Tapani Viitala *†
Published in: Langmuir, 29 (27), pp 8561–8571
A three-wavelength angular-scanning surface plasmon resonance based analysis has been utilized for characterizing optical properties of organic nanometer-thick layers with a wide range of thicknesses. [...]

Self-Defensive Biomaterial Coating Against Bacteria and Yeasts: Polysaccharide Multilayer Film with Embedded Antimicrobial Peptide

Publication year: 2013
Authors: Dr. G. Cado, L. Séon, Dr. T. Garnier, Dr. A. Parat,Dr. Y. Frère, Dr. L. Jierry, Prof. P. Schaaf, Dr. F. Boulmedais (1); R. Aslam, Dr. R. Fabre, A. Chassepot, Dr. J.-C. Voegel,Dr. B. Senger, Prof. P. Schaaf, Dr M.-H. Metz-Boutigue (2); Prof. F. Schneider (3); Dr. H. Kerdjoudj (4); Dr. L. Jierry, Prof. P. Schaaf, Dr. F. Boulmedais (5); Prof. P. Schaaf (6)
Published in: Advanced Functional Materials, 2013
Prevention of pathogen colonization of medical implants is a major medicaland fi nancial issue since infection by microorganisms constitutes one of the most serious complications after surgery or critical care. Immobilization ofantimicrobial molecules on biomaterials surfaces is an efficient approach to prevent biofilm formation. Herein, the first self-defensive coating against both bacteria and yeasts is reported where the release of the antimicrobial peptide is triggered by enzymatic degradation of the film due to the pathogens themselves. [...]

Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor

Publication year: 2013
Authors: Chang Zeng (a), Xin Huang (a), Jiangmin Xu (a), Guifen Li (a), Jie Ma (a), Hai-Feng Ji (b), Shuifang Zhu (a), Hongjun Chen (a)
Published in: Analytical Biochemistry, Vol. 440 (2013), p. 18–22
We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maizechlorotic mottle virus (MCMV). [...]

Water-Wettable Polypropylene Fibers by Facile Surface Treatment Based on Soy Proteins

Publication year: 2013
Authors: Salas C. 1,3, Genzer J. 2, Lucia L.A 1, Hubbe A.M. 1, Rojas O.J. 1,2, 4
Published in: ACS Applied Materials & Interfaces, 2013, Vol. 5, p. 6541 − 6548
Modification of the wetting behavior of hydrophobic surfaces is essential in a variety of materials, including textiles and membranes that require control of fluid interactions, adhesion, transport processes, sensing, etc. This investigation examines the enhancement of wettability of animportant class of textile materials, viz., polypropylene (PP) fibers, by surface adsorption of different proteins from soybeans, including soyflour, isolate, glycinin, and β-conglycinin. [...]

Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility

Publication year: 2013
Authors: H. G. Wang (1,2), T. Y. Yin (1,2), S. P. Ge (1,2), Q. Zhang (1,2), Q. L. Dong (1,2), D. X. Lei (1,2), D. M. Sun (1,2), G. X. Wang (1,2)
Published in: Journal of Biomedical Materials Research Part A, 2013, Vol. 101A, p. 413–420
In-stent restenosis and subsequent thrombosis remain a significant complication following the implantation of coronary stents. Different approaches have been used in developing novel coronary stents to protect against thrombosis and minimize restenosis. In the present study, we designed a biomacromolecular layer-by-layer coating with heparin, vascular endothelial growth factor (VEGF), and bronectin onto nickel-free titanium surface to improve blood compatibility and endothelial cell proliferation. [...]

Self-assembly of pyridine-modified lipoic Acid derivatives on gold and their interaction with thyroxine (t4)

Publication year: 2013
Authors: Albers WM, Milani R, Tappura K, Munter T, Resnati G, Metrangolo P.
Published in: International Journal of Molecular Sciences, 2013 Feb 6;14(2):3500-13.
Pyridyl derivatives of lipoic acid were prepared as ligands for the study of the interaction with thyroxine (T4). Thin self-assembled films of the ligands were prepared in 70% ethanol on gold and their interaction with T4 was studied by titration experiments in an aqueous buffer solution using Surface Plasmon Resonance (SPR). [...]

Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi

Publication year: 2013
Authors: D'Angelo G. 1, Uemura T., Chuang C.C., Polishchuk E., Santoro M., Ohvo-Rekilä H., Sato T., Di Tullio G., Varriale A., D'Auria S., Daniele T., Capuani F., Johannes L., Mattjus P., Monti M., Pucci P., Williams R.L., Burke J.E., Platt F.M., Harada A., De Matteis M.A.
Published in: Nature Research Letter, 2013, Vol. 501, p. 7465
Newly synthesized proteins and lipids are transported across the Golgi complex via different mechanisms whose respective roles are not completely clear. We previously identified a non-vesicular intra-Golgi transport pathway for glucosylceramide (GlcCer)—the common precursor of the different series of glycosphingolipids—that isoperated by the cytosolic GlcCer-transfer protein FAPP2 (also known as PLEKHA8). [...]

Real-Time Label-Free Monitoring of Drug- or Nanoparticle-Cell Interactions

Publication year: 2012
Authors: Tapani Viitala, Niko Granqvist, Huamin Liang, Marjo Yliperttula
Published in: Newsletter of the Controlled Release Society, 2012, Vol. 29, No. 6, p. 14-16
Efficient drug delivery is one of the major challenges in modern pharmaceutical research. There is a constant need for developing new formulation strategies for new drug delivery systems because new chemical entities tend to have low solubility, and biotechnology drugs (e.g., DNA, oligonucleotides, antibodies, and proteins) permeate poorly to the target sites, limiting or even prohibiting their therapeutic use. [...]

Structural and Functional Characteristics of Chimeric Avidins Physically Adsorbed onto Functionalized Polythiophene Thin Films, Albers et al. ACS Applied Materials & Interfaces

Publication year: 2012
Authors: Willem M. Albers †, Jani M. Pelto †, Clèment Suspenè ‡, Juha A.Määttä §, Abderrahim Yassar ⊥, Vesa P. Hytönen §#, Inger M.Vikholm-Lundin †, Kirsi Tappura †
Published in: ACS Applied Materials and Interfaces, 2012, Vol. 4, p. 4067 − 4077
Stabilized bioreceptor layers are of great importance in the design of novel biosensors. In earlier work, chimeric avidins enabled immobilization of biotinylated anti-bodies onto gold surfaces with greater stability compared to more conventional avidins (wild-type avidin and streptavidin). [...]

Kinetics of PKCe Activating and Inhibiting Llama Single Chain Antibodies and Their Effect on PKCe Translocation in HeLa Cells

Publication year: 2012
Authors: Milla Summanen 1,2, Niko Granqvist 3, Raimo K. Tuominen 2, Marjo Yliperttula 3, C.Theo Verrips 1, Johannes Boonstra 1; Christophe Blanchetot 1, Elina Ekokoski 2
Published in: PLos ONE, 2012, Vol. 7, Issue 4, e3563
Dysregulation of PKCe is involved in several serious diseases such as cancer, type II diabetes and Alzheimer’s disease.Therefore, specific activators and inhibitors of PKCe hold promise as future therapeutics, in addition to being useful inresearch into PKCe regulated pathways. We have previously described llama single chain antibodies (VHHs) that specificallyactivate (A10, C1 and D1) or inhibit (E6 and G8) human recombinant PKCe. [...]

Fluid dynamics modeling for synchronizing surface plasmon resonance and quartz crystal microbalance as tools for biomolecular and targeted drug delivery studies

Publication year: 2012
Authors: Tapani Viitala (a); Huamin Liang (a); Mayur Gupta (a), (b); Thomas Zwinger (c); Marjo Yliperttula (a); Alex Bunker (d); (e); ⇑
Published in: Journal of Colloid and Interface Science, Vol. 378 (2012), p. 251–259
We have used computational fluid dynamics modeling (CFD) to synchronize the flow conditions in the flow channels of two complementary surface-sensitive characterization techniques: surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). Since the footprint of the flow channels of the two devices is specified by their function, the flow behavior can only be varied either by altering the height ofthe flow channel, or altering the volumetric rate of flow (flow rate) through the channel. [...]

Surface plasmon resonance biosensor with high anti-fouling ability for the detection of cardiac marker troponin T

Publication year: 2011
Authors: J.T. Liu a, C.J. Chen b, T. Ikoma c, T. Yoshioka c, J.S. Cross d,e, S.-J.Chang a, J.-Z. Tsai b, J. Tanaka c
Published in: Analytica Chimica Acta, 2011, Vol. 703(1), p. 80-86
Designing a surface recognition layer with high anti-fouling ability, high affinity, and high specificity is an important issue to produce high sensitivity biosensing transducers. In this study, a self-assembled monolayer (SAM) consisting of a homogeneous mixture of oligo(ethylene glycol) (OEG)-terminated alkanethiolate and mercaptohexadecanoic acid (MHDA) on Au was employed for immobilizingtroponin T antibody and applied in detecting cardiac troponin T by using surface plasmon resonance (SPR). [...]

Modification of cellulose films by adsorption of CMC and chitosan for controlled attachment of biomolecules

Publication year: 2011
Authors: Hannes Orelma 1, Ilari Filpponen 2, Leena-Sisko Johansson 3, Janne Laine 4, Orlando Jose Rojas 5
Published in: Biomacromolecules, 2011, Vol. 12 (12), p. 4311–4318
The adsorption of human immunoglobulin G (hIgG) and bovine serum albumin (BSA) on cellulose supports were investigated. The dynamics and extent of related adsorption processes were monitored by surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D). Amine groups were installed on the cellulose substrate by adsorption of chitosan from aqueous solution, which allowed for hIgG to physisorb from acid media and produced a functionalized substrate with high surface density (10 mg/m2). [...]

Adsorption of PEO-PPO-PEO Triblock Copolymers with End-Capped Cationic Chains of Poly(2-dimethylaminoethyl methacrylate)

Publication year: 2011
Authors: Xiaomeng Liu†, Arja-Helena Vesterinen§, Jan Genzer‡, Jukka V. Seppälä§, and Orlando J. Rojas*†∥
Published in: Langmuir, 2011, Vol. 27 (16), p. 9769–9780
We study the adsorption of a symmetric triblockcopolymer of ethylene oxide, EO, and propylene oxide, PO,end-capped with quarternized poly(2-dimethylaminoethylmethacrylate), DMAEMA (DMAEMA24EO132PO50EO132DMAEMA24). Light scattering and tensiometry are used tomeasure the relative size of the associated structures and surfaceexcess at the airliquid interface. [...]

Surface Plasmon Resonance on Nanoscale Organic Films

Publication year: 2010
Authors: Willem M. Albers and Inger Vikholm-Lundin
Published in: Nano-Bio-Sensing, 1st ed, Chapter 4, Springer 2010
Surface Plasmon Resonance (SPR) has evolved over the past 20 years into a very versatile detection method, particularly in biosci- ence applications. Not only the scientific literature [...]

Surface plasmon resonance instrument as a refractometer for liquids and ultrathin films

Publication year: 2010
Authors: H.Liang a,b, H.Miranto b,c, N.Granqvist b,d, J.W. Sadowski c, T.Viitala b, B.Wang a, M.Yliperttula b
Published in: Sensors and Actuators B: Chemical 2010, 149(1), 212-220
A surface plasmon resonance (SPR) setup in Kretschmann configuration is being utilized as a refractometer for both liquids as well as ultrathin films. The SPR signal detection technology used is based on a goniometer approach providing a wide angular scan range which facilitates highly accurate liquid and gas phase measurements. [...]

Development of diagnostic SPR based biosensor for the detection of pharmaceutical compounds in saliva

Publication year: 2010
Authors: Susanna Sonny, Adama M. Sesay, Vesa Virtanen
Published in: Proc. SPIE 7376, Laser Applications in Life Sciences, 737605
The aim of the study is to develop diagnostic tests for the detection of pharmaceutical compounds in saliva. Oral fluid is increasingly being considered as an ideal sample matrix. It can be collected non-invasively and causes less stress to the person being tested. [...]