Search publications 


Self-assembly of flagellin on Au(111) surfaces

Publication year: 2014
Authors: González Orive A. 1, Pissinis D.E. 2, Diaz C. 2, Miñán A. 2, Benítez G.A. 2, Rubert A. 2, Daza Millone A. 2, Rumbo M. 3, Hernández Creus A. 1, Salvarezza R.C. 2, Schilardi P.L. 4
Published in: Journal of Colloid and Interface Science, Vol. 433 (2014), p. 86–93
The adsorption of flagellin monomers from Pseudomonas fluorescens on Au(111) has been studied by Atomic Force Microscopy (AFM), Scanning Tunneling Microscopy (STM), X-ray Photoelectron Spectroscopy (XPS), Surface Plasmon Resonance (SPR), and electrochemical techniques. [...]

Dynamic and equilibrium performance of sensors based on short peptide ligands for affinity adsorption of human IgG using surface plasmon resonance

Publication year: 2014
Authors: Islam N. 1, Shen F. 1, Gurgel P.V. 1, Rojas O.J. 2, Carbonell R.G. 3
Published in: Biosensors and Bioelectronics, Vol. 58, 2014, p. 380-387
This paper characterizes the potential of novel hexameric peptide ligands for on-line IgG detection in bioprocesses. Surface Plasmon Resonance (SPR) was used to study the binding of human IgG to the hexameric peptide ligand HWRGWV, which was covalently grafted to alkanethiol self-assembled monolayers (SAM) on gold surfaces. [...]

Control of the Morphology of Lipid Layers by Substrate Surface Chemistry

Publication year: 2014
Authors: Niko Granqvist *†, Marjo Yliperttula †, Salla Välimäki ‡, Petri Pulkkinen ‡, Heikki Tenhu ‡, and Tapani Viitala *†
Published in: Langmuir, 2014, Vol. 30 (10), p. 2799–2809
In this study, surface coatings were used to control the morphology of the deposited lipid layers during vesicle spreading, i.e., to control if liposomes self-assemble on a surface into a supported lipid bilayer or a supported vesicular layer. [...]

Effect of gold nanoparticles on the structure and electron-transfer characteristics of glucose oxidase redox polyelectrolyte-surfactant complexes

Publication year: 2014
Authors: Cortez M.L. a,b, Marmisollé W. a, Pallarola D. b, Pietrasanta L.I. c, Murgida D.H. a, Ceolín M. b, Azzaroni O. b, Battaglini F. a
Published in: Chemistry A European Journal, 2014, Vol. 20, Issue 41, p. 13037–13404
Efficient electrical communication between redox proteins and electrodes is a critical issue in the operation and development of amperometric biosensors. The present study explores the advantages of a nanostructured redox-active polyelectrolyte-surfactant complex containing [Os(bpy)2Clpy](2+) (bpy=2,2'-bipyridine, py= pyridine) as the redox centers and gold nanoparticles (AuNPs) as nanodomains for boosting the electron-transfer propagation throughout the assembled film in the presence of glucose oxidase (GOx). [...]

Poly(l-lactide-co-2-(2-methoxyethoxy)ethyl methacrylate): A biodegradable polymer with protein resistance

Publication year: 2014
Authors: Jinbao Xua (a), Xuelin Fan (b), Jinxian Yang (b), Chunfeng Ma (a),Xiaodong Ye (b), Guangzhao Zhanga,(b)
Published in: Colloids and Surfaces B: Biointerfaces, Vol. 116 (2014), p. 531–536
We have synthesized poly(l-lactide-co-2-(2-methoxyethoxy)ethyl methacrylate) (LA-co-MEO2MA) containing both degradable and protein resistant units via hybrid copolymerization with(1-tert-butyl-4,4,4-tris(dimethylamino)-2,2-bis[tris(dimethylamino)phophoranylidenamino]-2Λ5, Λ5-catenadi(phosphazene) (t-BuP4) as the catalyst. [...]

Study of the interactions of proteins with a solid surface using complementary acoustic and optical techniques

Publication year: 2014
Authors: Diaconu G1, Schäfer T1
Published in: Biointerphases 9, 029015 (2014)
Membrane water treatment processes suffer severely from (bio)fouling phenomena, defined as an undesired deposition and build-up of adsorbed materials, which alters the membrane performance. [...]

Affibody conjugation onto bacterial cellulose tubes and bioseparation of human serum albumin

Publication year: 2014
Authors: Hannes Orelma,*a Luis O. Morales,a Leena-Sisko Johansson,a Ingrid C. Hoeger,b Ilari Filpponen,a Cristina Castro,c Orlando J. Rojasab and Janne Lainea
Published in: RSC Adv., 2014,4, p. 51440-51450
We attached anti-human serum albumin (anti-HSA) affibody ligands on bacterial cellulose (BC) by EDC–NHS-mediated covalent conjugation and physical adsorption and demonstrate their application for tubular biofiltration of blood proteins. [...]

Using gelatin protein to facilitate paper thermoformability

Publication year: 2014
Authors: A. Khakalo a, I. Filpponen a, L.S. Johansson a, A. Vishtal b, A.R. Lokanathan a,O. J. Rojas a,c, J. Laine a
Published in: Reactive & Functional Polymers, 2014
One of the main challenges of fiber-based packaging materials is the relatively poor elongation of cellulose under stress, which limits formability and molding in related products. [...]

Biodegradable Polyurethane Carrying Antifoulants for Inhibition of Marine Biofouling

Publication year: 2014
Authors: Jielin Ma †, Chunfeng Ma *†, Yun Yang †, Wentao Xu †, and Guangzhao Zhang *†‡
Published in: Industrial & Engineering Chemistry Research, 2014, 53 (32), p. 12753–12759
Biodegradable polyurethane with N-(2,4,6-trichlorophenyl)maleimide (TCPM) pendant groups has been prepared via a combination of a thiol–ene click reaction and a condensation reaction. [...]

Tuning Cellulose Nanocrystal Gelation with Polysaccharides and Surfactants

Publication year: 2014
Authors: Zhen Hu, Emily D. Cranston, Robin Ng, and Robert Pelton*
Published in: Langmuir, 2014, 30 (10), pp 2684–2692
Gelation of cellulose nanocrystal (CNC) dispersions was measured as a function of the presence of four nonionic polysaccharides. Addition of hydroxyethyl cellulose (HEC), hydroxypropyl guar (HPG), or locust bean gum (LBG) to CNC dispersions [...]

Surface modification of cellulose nanocrystals with cetyltrimethylammonium bromide

Publication year: 2014
Authors: Tiffany Abitbol, Heera Marway and, Emily D. Cranston Tiffany Abitbol (, Heera Marway (, Emily D. Cranston (
Published in: Special Issue: NANOCELLULOSE; Nordic Pulp & Paper Research Journal Vol 29 no (1) 2014
Cellulose nanocrystals (CNCs) prepared by sulfuric acid hydrolysis of cotton were surface modified with cetyltrimethylammonium bromide (CTAB). Essentially, the counterions of the CNC surface sulfate ester groups are exchanged for cetyltrimethyl-ammonium (CTA+), which acts as a bulky, amphiphilic cation. [...]

Photoresponsive cellulose fibers by surface modification with multifunctional cellulose derivatives

Publication year: 2014
Authors: Olga Grigoray (a), Holger Wondraczek(a), Elina Heikkilä(a), Pedro Fardim (a,b,∗), Thomas Heinze (a,c)
Published in: Carbohydrate Polymers 111 (2014) 280–287
Eucalyptus bleached kraft pulp fibers were modified by adsorption of novel bio-based multifunctionalcellulose derivatives in order to generate light responsive surfaces. [...]

Modification of surfaces with adsorption of amphiphilic polymers

Publication year: 2014
Authors: Katri Kontturi
Published in: Doctoral Dissertation
In this work the adsorption of amphiphilic polymers and their utilization in the modification of planar surfaces were examined. The approach was to systematically explore adsorption of very different types of [...]

Specificity and regenerability of short peptide ligands supported on polymer layers for immunoglobulin G binding and detection

Publication year: 2013
Authors: Zhang Y. 1, Islam N. 2, Carbonell R.G. 2, Rojas O.J. 1,2
Published in: ACS Applied Materials and Interfaces, 2013, Vol.5 (16), p. 8030-7
We demonstrate the specificity, regenerability, and excellent storage stability of short peptide-based systems for detection of immunoglobulin G (IgG). The bioactive component consisted of [...]

Non-labelled surface sensitive techniques as platforms for pharmaceutical nanotechnology research

Publication year: 2013
Authors: Huamin Liang, 2013
Published in: Doctor's Dissertation (article-based)
Insufficient delivery of drugs to the target sites like tumors and cells has been a barrier for achieving satisfying therapeutic effects in many diseases. Distribution and exposure of drugs to normal and healthy tissues may enhance the possibility of side effects and toxicity in vivo. Nanoparticle (NP) drug delivery systems have been developed to enable targeting of drugs to target sites and at the same time also reduce or even eliminate the distribution and exposure of drugs to non-targeted sites (normal and healthy tissues). The interactions of ligand attached NPs with specific receptors on the cell surface enable intracellular delivery of drugs. [...]

Specific water uptake of thin films from nanofibrillar cellulose

Publication year: 2013
Authors: Katri S. Kontturi (a), Eero Kontturi (a), Janne Laine (a)
Published in: J.Mater. Chem. A, 2013
Swelling of NFC films was surveyed by quartz crystal microbalance with dissipation monitoring coupled with H2O/D2O exchange and surface plasmon resonance. The influence of charged groups on swelling was investigated by neutralizing the charge of NFC with cationic polyelectrolytes or increasing the charge with carboxymethyl cellulose (CMC). [...]

Grafting from Poly(3,4-ethylenedioxythiophene): A Simple Route to Versatile Electrically Addressable Surfaces

Publication year: 2013
Authors: Jenny Malmström *†‡, Michel K Nieuwoudt †, Lisa T Strover †‡, Alissa Hackett †, Olivia Laita †, Margaret A Brimble †, David E Williams †‡, and Jadranka Travas-Sejdic *†‡
Published in: Macromolecules, 2013, 46 (12), pp 4955–4965
We demonstrate a simple route to versatile electrically addressable conductive polymer graft copolymer systems. [...]

Specific binding of immunoglobulin G with bioactive short peptides supported on antifouling copolymer layers for detection in quartz crystal microgravimetry and surface plasmon resonance

Publication year: 2013
Authors: Yanxia Zhang †, Nafisa Islam ‡, Ruben G. Carbonell ‡, Orlando J. Rojas †‡
Published in: Analytical Chemistry, Vol. 85, Issue 2, 2013, p. 1106–1113
A new peptide-based system supported on copolymer brushes grafted from gold sensors and with resistance to nonspecific adsorption is reported for selective binding of human immunoglobulin G (IgG). [...]

Hybrid POSS-Containing Brush on Gold Surfaces for Protein Resistance

Publication year: 2013
Authors: Xiaolu Ye (a), Junbo Gong (b), Zhongping Wang (b), Zengming Zhang (b), Sheng Han (c), Xuesong Jiang (a)*
Published in: Macromol. Biosci. 2013, 13, 921–926
A hybrid polymer brush containing poly(ethylene glycol) (PEG) chains and polyhedraloligosilsesquioxane (POSS) on a gold surface is presented that exhibits an excellent protein resistance and long-term stability. [...]

Elucidating the Signal Responses of Multi-Parametric Surface Plasmon Resonance Living Cell Sensing: A Comparison between Optical Modeling and Drug–MDCKII Cell Interaction Measurements

Publication year: 2013
Authors: Tapani Viitala 1, Niko Granqvist 1, Susanna Hallila 1, Marjo Yliperttula 1, Manuela Raviña 2
Published in: PLoS ONE, 2013, Vol. 8(8), e72192
In vitro cell-based assays are widely used during the drug discovery and development process to test the biological activity of new drugs. Most of the commonly used cell-based assays, however, lack the ability to measure in real-time or under dynamic conditions (e.g. constant flow). In this study a multi-parameter surface plasmon resonance approach in combination with living cell sensing has been utilized for monitoring drug-cell interactions in real-time, under constant flow and without labels. [...]