MP-SPR for living cells

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There are many drivers behind cell-based research including tissue engineering, in vitro toxicology, drug delivery, but also antibacterial coatings.

Surface plasmon resonance has been originally commercialized for protein interaction measurements. It is only recently that researchers started developing the SPR for measurements on living cells. We are proud to be the first ones to enable measurements of trans- and paracellular uptake. 

 

  • BioNavis together with University of Helsinki is pioneering the measurements of drug- and nanoparticle-cell interactions
  • Many of our users are using MP-SPR to measure cell spreading or cell attachment on different biomaterials and coatings
  • MP-SPR can also be used as a biosensor in detection of cell-specific metabolites to confirm cell differentiation and/or cell functionality
  • Similarly, a few groups are working on biofilm formation or antibacterial coatings

See five reasons to choose MP-SPR for living cells:

  • Able to measure living cell and bacteria attachment in real-time
  • Able to separate permeation from internalization label-free
  • Surface freedom
  • Control of temperature and shear-stress
  • Combination of MP-SPR with electrochemistry

See five key questions about living cells that MP-SPR can answer:

  • What is drug absorption route of this pharmaceutical?
  • Which nanoparticle is the best one for drug delivery?
  • How does a nanoparticle or virus enter the cell?
  • What is the kinetics of cell attachment to a surface? 
  • Which surface is the most resistant to bacterial growth?

See selected Application Notes to see how MP-SPR works with living cells:

  • AN#156 Nanoparticle uptake by cells measured using MP-SPR
  • AN#145 Virus interaction studies using MP-SPR
  • AN#137 Drug - living cell interaction
  • AN#154 Real-time cancer cell detection and cell adhesion on implant materials surface

 See the animation of drug-cell interaction measured with MP-SPR!


Selected publications:

What our users say about MP-SPR for living cell measurements:

“MP-SPR allows us to work with living cell monolayers grown directly on the sensor surface or with the aid of e.g. fibronectin and other growth promoting proteins. With MP-SPR, we are able to observe and quantify the differences in cell uptake kinetics of nanoparticles in dependence with the surface characteristics of the nanoparticle and their targeting." - Asst. Prof. Tapani Viitala, University of Helsinki, Finland


 


 

See five reasons to choose MP-SPR for living cells studies.