SPR Navi™ is especially useful in the research of

Biosensors Protein bindings Antibody/antigen interactions Drug discovery Gas sensors Pathogen detection in food Environmental monitoring Protein drug research Drug screening Food quality testing Enviromental pollutants testing Antibody screening Coating compatibility Gas sensing Basic research of molecular interactions dNA screening Biomolecular assay development

Application highlights:

AN117 Quantitative detection of genetic material

dNA assays can be applied for detecting genetic material in order to detect genetic disorders, mutations, gene transfection or species from a large variety of samples. By assaying specific single stranded oligonucleotides of over 20 nucleotides in length, it is possible to detect and quantify unique gene sequences from large amounts of genetic material. BioNavis SPR Navi™ instruments can be utilized easily into a dNA assay that can detect and quantify single-stranded oligonucleotides with high precision over a large concentration range from micro to nanomolar concentrations.

"Quantification of single stranded oligonucleotides with SPR Navi 200" Compilation of the sensograms from several different injections (upper graph) First order exponential fit to the bound amount of dNA (lower graph)

Full Application Note

AN109 LB film characterization in air

Creating ordered single or multiple layers of molecules has a multitude of technology applications in, for instance, biosensors and nanoelectronics etc. In this example, Langmuir-Blodgett deposition was used to deposit single and multiple layers of stearic acid on gold. By measuring the full SPR intensity versus angle curves in air, the layer thickness after successive depositions could be quickly and easily confirmed. In this case, the film thickness increased linearly with the number of layers.

"Precise control of Langmuir-Blodgett film thickness" SPR intensity versus angle curves measured in air for an increasing number of LB layers of Stearic Acid on gold.

Full Application Note

AN110 Albumin adsorption

A typical application in biomaterials research is the adsorption of protein to tailored surfaces. In this case, SPR Navi™ instrument was used to follow the adsorption of 0.1 mg/mL Bovine Serum Albumin (BSA) onto a gold surface before and after self-assembly of an alkanethiol. The results show that hydrophobization of the gold halved the amount of albumin adsorbed. The layer thickness and adsorbed amount can be determined in each case, by fitting the measured data with theory.

"Highly sensitive measurements of protein adsorption on gold" Change in SPR signal during BSA adsorption to hydrophilic and hydrophobic gold.

Full Application Note

AN113 Quantitative Detection of Small Molecules

BioNavis SPR Navi™ instrument was shown to have excellent performance with challenging small molecular weight samples, such as Dopamine. The high amount of non-specific interactions during injections did not affect the quantitative performance of the instrument. The method described can be easily adopted for other biochemical assays where low concentrations of small molecular weight analysts need to be quantified.

"Quantitative Detection of Dopamine" Example of sensogram (upper graph) Signal dependency on dopamine concentration (lower graph)

Full Application Note

AN114 Precisely characterizing molecular interactions kinetics without chemical labeling

Two experiments demonstrate that the SPR Navi™ instruments are sensitive for studies of biomolecular kinetics. It enables precise application of kinetic models including associa-tion and dissociation constants. The SPR Navi™ instruments are suitable for both large dynamic range and high sensitivity experiments. The open design of the SPR Navi™ instruments allow easy measurement on biochemical surfaces prepared outside the instrument, allowing efficient optimization of the surface chemistry of the sensor slide for biochemical studies.

"Biomolecular interaction kinetics measured using Surface Plasmon Resonance" A folic acid – folate binding protein injection with on-line referencing. The upper curve is the sample channel and the lower curve isthe reference channel.

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